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dc.contributor.authorTenorio, M. S.-
dc.contributor.authorOliveira e Sousa, L.-
dc.contributor.authorAlves-Martin, M. F.-
dc.contributor.authorPaixao, M. S.-
dc.contributor.authorRodrigues, M. V.-
dc.contributor.authorStarke-Buzetti, W. A.-
dc.contributor.authorAraujo Junior, J. P.-
dc.contributor.authorLucheis, Simone Baldini-
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier Science Bv, v. 203, n. 1-2, p. 203-206, 2014.-
dc.description.abstractDiverse wild animal species can be reservoirs of zoonotic flagellate parasites, which can cause pathologic Chagas disease. The present study aimed to detect the natural occurrence of flagellate parasites through direct microscopic examination of the Parasites in blood samples and through PCR of whole blood and blood culture (haemoculture) samples from 38 captive and 65 free-living wild animals in the Centre for Conservation of Wild Fauna (CCWF), an area endemic for leishmaniasis. For this study, PCR was accomplished using primers for the ribosomal region (ITS-1) of the flagellate parasites. The amplified fragments were cloned and sequenced to identify DNA of the Trypanosomatid parasite species, observed in blood cultures from 3.9% (04/103) of the animals. Through these techniques, Trypanosoma cruzi was identified in haemoculture samples of the following three free-living species: common agouti (Dasyprocta aguti), white-eared opossum (Didelphis albiventris), and nine-banded armadillo (Dasypus novemcinctus). Furthermore, Trypanosoma minasense was identified in whole blood samples from 01 (0.9%) captive animal (black howler monkey-Alouatta caraya). These results demonstrated the first report of T. cruzi isolation in wild species from the CCWF using blood culture, which can be applied in addition to molecular tools for epidemiological studies and to identify trypanosomatids in wild animals. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipCentre for Conservation of Wild Fauna (CCWF), Sao Paulo, Brazil-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectTrypanosoma cruzien
dc.subjectWild animalsen
dc.titleMolecular identification of trypanosomatids in wild animalsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionCtr Conservat Wild Fauna-
dc.contributor.institutionPaulista Agcy Agribusiness Technol-
dc.description.affiliationIBB UNESP, Dept Microbiol & Immunol, BR-18618970 Sao Paulo, Brazil-
dc.description.affiliationCtr Conservat Wild Fauna, BR-15385000 Sao Paulo, Brazil-
dc.description.affiliationFMB UNESP, Dept Trop Dis, BR-18618970 Sao Paulo, Brazil-
dc.description.affiliationFEIS UNESP, Dept Biol & Anim Husb, BR-15385000 Sao Paulo, Brazil-
dc.description.affiliationPaulista Agcy Agribusiness Technol, APTA, BR-17030000 Sao Paulo, Brazil-
dc.description.affiliationUnespIBB UNESP, Dept Microbiol & Immunol, BR-18618970 Sao Paulo, Brazil-
dc.description.affiliationUnespFMB UNESP, Dept Trop Dis, BR-18618970 Sao Paulo, Brazil-
dc.description.affiliationUnespFEIS UNESP, Dept Biol & Anim Husb, BR-15385000 Sao Paulo, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofVeterinary Parasitology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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