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DC Field | Value | Language |
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dc.contributor.author | Tanni, Suzana E. | - |
dc.contributor.author | Corrêa, Camila Renata | - |
dc.contributor.author | Angeleli, Aparecida Y. | - |
dc.contributor.author | Vale, Simone A. | - |
dc.contributor.author | Coelho, Liana S. | - |
dc.contributor.author | Godoy, Irma de | - |
dc.date.accessioned | 2014-05-20T13:33:13Z | - |
dc.date.available | 2014-05-20T13:33:13Z | - |
dc.date.issued | 2012-11-21 | - |
dc.identifier | http://dx.doi.org/10.1186/1476-9255-9-45 | - |
dc.identifier.citation | Journal of Inflammation-london. London: Biomed Central Ltd., v. 9, p. 6, 2012. | - |
dc.identifier.issn | 1476-9255 | - |
dc.identifier.uri | http://hdl.handle.net/11449/11352 | - |
dc.description.abstract | Background: Smoking is known to be associated with oxidative stress; however, it has not been elucidated whether the oxidative response is influenced by the intensity of smoking exposure.Objectives: Evaluate the effect of smoking exposure on the secretion of hydrogen peroxide (H2O2) by the peripheral blood monocytes of smokers.Methods: A total of 25 smokers (50.3 +/- 8.8 years, 48% male) underwent the following evaluations: spirometry, pulse oximetry, body composition and total peripheral blood count. Peripheral blood monocyte (PBM) cultures were isolated and maintained, and IL-6 and TNF-alpha were measured in the plasma and in the supernatants of spontaneous and stimulated cultures. H2O2 was evaluated in the supernatants of the PBM cultures, and a subset of the PBM culture supernatants was stimulated with phorbol myristate acetate (PMA). We also evaluated 38 healthy controls (49.1 +/- 8.2 years, 42% male).Results: The spontaneous and stimulated monocytes' secretion of H2O2 were statistically higher in the smokers than in the healthy controls (p<0.001). The H2O2 secretions were statistically significant higher after stimulation with PMA in both groups (p<0.001). In the multiple regression analysis, we identified a positive, statistically significant association between pack-years of smoking and the spontaneous secretion of H2O2 by PBM culture, adjusted for potential confounding variables. The association between PBM culture secretion of H2O2 and the production of TNF-alpha and IL-6 was not significant.Conclusion: We identified a positive association between higher production of H2O2 in smokers and higher smoking exposure during life. The influence of pack-years smoking may be a key modifiable factor in oxidative stress associated to smoking. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.format.extent | 6 | - |
dc.language.iso | eng | - |
dc.publisher | Biomed Central Ltd. | - |
dc.source | Web of Science | - |
dc.subject | Hydrogen peroxide | en |
dc.subject | Smoking | en |
dc.subject | Oxidative stress | en |
dc.subject | Systemic inflammation | en |
dc.subject | Cultured cells | en |
dc.title | Increased production of hydrogen peroxide by peripheral blood monocytes associated with smoking exposure intensity in smokers | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ Estadual Paulista, UNESP, Disciplina Pneumol, Fac Med Botucatu, São Paulo, Brazil | - |
dc.description.affiliation | UNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista, UNESP, Dept Patol, Fac Med Botucatu, São Paulo, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, UNESP, Disciplina Pneumol, Fac Med Botucatu, São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, UNESP, Dept Patol, Fac Med Botucatu, São Paulo, Brazil | - |
dc.description.sponsorshipId | FAPESP: 03/05285-1 | - |
dc.identifier.doi | 10.1186/1476-9255-9-45 | - |
dc.identifier.wos | WOS:000312597300001 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | WOS000312597300001.pdf | - |
dc.relation.ispartof | Journal of Inflammation-london | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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