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http://acervodigital.unesp.br/handle/11449/117393
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DC Field | Value | Language |
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dc.contributor.author | Corniani, Natalia | - |
dc.contributor.author | Velini, Edivaldo Domingues | - |
dc.contributor.author | Silva, Ferdinando Marcos Lima | - |
dc.contributor.author | Nanayakkara, N. P. Dhammika | - |
dc.contributor.author | Witschel, Matthias | - |
dc.contributor.author | Dayan, Franck E. | - |
dc.date.accessioned | 2015-03-18T15:56:01Z | - |
dc.date.accessioned | 2016-10-25T20:35:15Z | - |
dc.date.available | 2015-03-18T15:56:01Z | - |
dc.date.available | 2016-10-25T20:35:15Z | - |
dc.date.issued | 2014-07-31 | - |
dc.identifier | http://dx.doi.org/10.1371/journal.pone.0103704 | - |
dc.identifier.citation | Plos One. San Francisco: Public Library Science, v. 9, n. 7, 10 p., 2014. | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/11449/117393 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/117393 | - |
dc.description.abstract | The 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway leads to the synthesis of isopentenyl diphosphate in plastids. It is a major branch point providing precursors for the synthesis of carotenoids, tocopherols, plastoquinone and the phytyl chain of chlorophylls, as well as the hormones abscisic acid and gibberellins. Consequently, disruption of this pathway is harmful to plants. We developed an in vivo bioassay that can measure the carbon flow through the carotenoid pathway. Leaf cuttings are incubated in the presence of a phytoene desaturase inhibitor to induce phytoene accumulation. Any compound reducing the level of phytoene accumulation is likely to interfere with either one of the steps in the MEP pathway or the synthesis of geranylgeranyl diphosphate. This concept was tested with known inhibitors of steps of the MEP pathway. The specificity of this in vivo bioassay was also verified by testing representative herbicides known to target processes outside of the MEP and carotenoid pathways. This assay enables the rapid screen of new inhibitors of enzymes preceding the synthesis of phytoene, though there are some limitations related to the non-specific effect of some inhibitors on this assay. | en |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
dc.format.extent | 10 | - |
dc.language.iso | eng | - |
dc.publisher | Public Library Science | - |
dc.source | Web of Science | - |
dc.title | Novel bioassay for the discovery of inhibitors of the 2-C-Methyl-D-erythritol 4-Phosphate (MEP) and terpenoid pathways leading to carotenoid biosynthesis | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Univ Mississippi | - |
dc.contributor.institution | BASF SE | - |
dc.contributor.institution | USDA ARS | - |
dc.description.affiliation | Sao Paulo State Univ, Fac Agron Sci, Botucatu, SP, Brazil | - |
dc.description.affiliation | Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, University, MS 38677 USA | - |
dc.description.affiliation | BASF SE, GVA HC B009, Ludwigshafen, Germany | - |
dc.description.affiliation | USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA | - |
dc.description.affiliationUnesp | Sao Paulo State Univ, Fac Agron Sci, Botucatu, SP, Brazil | - |
dc.description.sponsorshipId | CAPES: BEX 0226/12-2 | - |
dc.identifier.doi | 10.1371/journal.pone.0103704 | - |
dc.identifier.wos | WOS:000339954800066 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | WOS000339954800066.pdf | - |
dc.relation.ispartof | Plos One | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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