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dc.contributor.authorLombardo Bedran, Telma Blanca-
dc.contributor.authorAlves Mayer, Marcia Pinto-
dc.contributor.authorSpolidorio, Denise Palomari-
dc.contributor.authorGrenier, Daniel-
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 9, 10 p., 2014.-
dc.description.abstractGiven the spread of antibiotic resistance in bacterial pathogens, antimicrobial peptides that can also modulate the immune response may be a novel approach for effectively controlling periodontal infections. In the present study, we used a three-dimensional (3D) co-culture model of gingival epithelial cells and fibroblasts stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS) to investigate the anti-inflammatory properties of human beta-defensin-3 (hBD-3) and cathelicidin (LL-37) and to determine whether these antimicrobial peptides can act in synergy. The 3D co-culture model composed of gingival fibroblasts embedded in a collagen matrix overlaid with gingival epithelial cells had a synergistic effect with respect to the secretion of IL-6 and IL-8 in response to LPS stimulation compared to fibroblasts and epithelial cells alone. The 3D co-culture model was stimulated with non-cytotoxic concentrations of hBD-3 (10 and 20 mu M) and LL-37 (0.1 and 0.2 mu M) individually and in combination in the presence of A. actinomycetemcomitans LPS. A multiplex ELISA assay was used to quantify the secretion of 41 different cytokines. hBD-3 and LL-37 acted in synergy to reduce the secretion of GRO-alpha, G-CSF, IP-10, IL-6, and MCP-1, but only had an additive effect on reducing the secretion of IL-8 in response to A. actinomycetemcomitans LPS stimulation. The present study showed that hBD-3 acted in synergy with LL-37 to reduce the secretion of cytokines by an LPS-stimulated 3D model of gingival mucosa. This combination of antimicrobial peptides thus shows promising potential as an adjunctive therapy for treating inflammatory periodontitis.en
dc.description.sponsorshipCanadian Institutes of Health Research-
dc.description.sponsorshipLaboratoire de Controle Microbiologique de l'Universite Laval-
dc.publisherPublic Library Science-
dc.sourceWeb of Science-
dc.titleSynergistic Anti-Inflammatory Activity of the Antimicrobial Peptides Human Beta-Defensin-3 (hBD-3) and Cathelicidin (LL-37) in a Three-Dimensional Co-Culture Model of Gingival Epithelial Cells and Fibroblastsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniv Laval-
dc.description.affiliationState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Sao Paulo, Brazil-
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, Brazil-
dc.description.affiliationState Univ Sao Paulo, Araraquara Dent Sch, Dept Physiol & Pathol, Sao Paulo, Brazil-
dc.description.affiliationUniv Laval, Fac Dent, Oral Ecol Res Grp, Quebec City, PQ, Canada-
dc.description.affiliationUnespState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, Sao Paulo, Brazil-
dc.description.affiliationUnespState Univ Sao Paulo, Araraquara Dent Sch, Dept Physiol & Pathol, Sao Paulo, Brazil-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofPlos One-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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