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Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes
  • Universidade Estadual Paulista (UNESP)
  • Cornell Univ
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Sponsorship Process Number: 
  • FAPESP: 08/55417-5
  • FAPESP: 08/55517-0
Objective: To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis.Study design: A case control study that included 42 pregnant women not in labor in the following groups: PPROM (n = 28) and controls with intact membranes submitted to selective cesarean section at term (n = 14). Expression of IL-18 mRNA in chorioamniotic membranes was determined by real-time polymerase chain reaction, and IL-18 protein expression was measured by western blot. Histopathological analyses and immunolocalization of IL-18 by immunohistochemistry were also performed. Analyses were performed using the Mann-Whitney or Fisher's exact tests and the group effect was considered significant if the adjusted p-values were <0.05 and the magnitude of change was greater than 2-fold for mRNA expression.Results: IL-18 mRNA was present in 100% of samples and no difference in expression was observed between term vs. PPROM membranes (fold-change 0.12; p = 0.88). In the PPROM group, no difference was observed in IL-18 mRNA regarding gestational age (fold-change 0.11; p = 0.42) or the presence of histological chorioamnionitis (fold-change 0.26; p = 0.15). ProIL-18 was present in all samples. IL-18 was immunolocalized to amnion, chorion and decidua cells, with intense immunohistochemical staining at the choriodecidual junction.Conclusion: Chorioamniotic membranes are sources of IL-18 mRNA and proIL-18, and their expression is unrelated to PPROM or histological chorioamnionitis.
Issue Date: 
European Journal of Obstetrics & Gynecology and Reproductive Biology. Amsterdam: Elsevier B.V., v. 161, n. 2, p. 134-139, 2012.
Time Duration: 
Elsevier B.V.
  • Preterm prelabor rupture of membranes
  • Interleukin-18
  • proIL-18
  • Histological chorioamnionitis
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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