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DC Field | Value | Language |
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dc.contributor.author | Polettini, Jossimara | - |
dc.contributor.author | Vieira, Eliane Passarelli | - |
dc.contributor.author | Santos, Mariana Perlati dos | - |
dc.contributor.author | Peraçoli, José Carlos | - |
dc.contributor.author | Witkin, Steven Sol | - |
dc.contributor.author | Silva, Marcia da | - |
dc.date.accessioned | 2014-05-20T13:35:15Z | - |
dc.date.available | 2014-05-20T13:35:15Z | - |
dc.date.issued | 2012-04-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.ejogrb.2011.12.026 | - |
dc.identifier.citation | European Journal of Obstetrics & Gynecology and Reproductive Biology. Amsterdam: Elsevier B.V., v. 161, n. 2, p. 134-139, 2012. | - |
dc.identifier.issn | 0301-2115 | - |
dc.identifier.uri | http://hdl.handle.net/11449/12112 | - |
dc.description.abstract | Objective: To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis.Study design: A case control study that included 42 pregnant women not in labor in the following groups: PPROM (n = 28) and controls with intact membranes submitted to selective cesarean section at term (n = 14). Expression of IL-18 mRNA in chorioamniotic membranes was determined by real-time polymerase chain reaction, and IL-18 protein expression was measured by western blot. Histopathological analyses and immunolocalization of IL-18 by immunohistochemistry were also performed. Analyses were performed using the Mann-Whitney or Fisher's exact tests and the group effect was considered significant if the adjusted p-values were <0.05 and the magnitude of change was greater than 2-fold for mRNA expression.Results: IL-18 mRNA was present in 100% of samples and no difference in expression was observed between term vs. PPROM membranes (fold-change 0.12; p = 0.88). In the PPROM group, no difference was observed in IL-18 mRNA regarding gestational age (fold-change 0.11; p = 0.42) or the presence of histological chorioamnionitis (fold-change 0.26; p = 0.15). ProIL-18 was present in all samples. IL-18 was immunolocalized to amnion, chorion and decidua cells, with intense immunohistochemical staining at the choriodecidual junction.Conclusion: Chorioamniotic membranes are sources of IL-18 mRNA and proIL-18, and their expression is unrelated to PPROM or histological chorioamnionitis. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.format.extent | 134-139 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Preterm prelabor rupture of membranes | en |
dc.subject | Interleukin-18 | en |
dc.subject | proIL-18 | en |
dc.subject | Histological chorioamnionitis | en |
dc.title | Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Cornell Univ | - |
dc.description.affiliation | UNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliation | UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliation | Cornell Univ, Dept Obstet & Gynecol, Weill Med Coll, New York, NY 10021 USA | - |
dc.description.affiliationUnesp | UNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.sponsorshipId | FAPESP: 08/55417-5 | - |
dc.description.sponsorshipId | FAPESP: 08/55517-0 | - |
dc.identifier.doi | 10.1016/j.ejogrb.2011.12.026 | - |
dc.identifier.wos | WOS:000302765400004 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | WOS000302765400004.pdf | - |
dc.relation.ispartof | European Journal of Obstetrics & Gynecology and Reproductive Biology | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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