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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/122593
Título: 
Production of β-(1,3)-glucanases by Trichoderma harzianum Rifai: Optimization and Application to Produce Gluco-oligosaccharides from Paramylon and Pustulan
Autor(es): 
Instituição: 
Universidade Estadual Paulista (UNESP)
ISSN: 
2167-7972
Resumo: 
β-(1→3)-Glucanases were produced by Trichoderma harzianum Rifai PAMB-86 cultivated on botryosphaeran in a bench-fermenter and optimised by the response surface method. Maximal enzyme titres occurred at 5 days, initial pH 5.5 and aeration of 1.5vvm. β-(1→3)-The β-glucanolytic enzyme complex produced by T. harzianum Rifai PAMB- 86 was fractionated by gel filtration into 2 fractions (F-I, F-II), and employed to produce gluco-oligosaccharides from algal paramylon ((1→3)-β-D-glucan) and lichen pustulan ((1→6)-β-D-glucan). Both enzymes attacked paramylon to the extent of ~15-20% in 30 min releasing glucose and laminaribiose as major end-products, and laminarioligosaccharides of degree of polymerization (DP) ≥3. Only F-I degraded pustulan resulting in ~2% degradation at 30 min, with glucose, gentiobiose and gentio-oligosaccharides of DP ≥4 as major products. The difference in the nature of the hydrolysis products can be explained by the substrate specificities of each enzyme fraction, and the structural differences of the β-D-glucans attacked.
Data de publicação: 
2011
Citação: 
Fermentation Technology, v. 1, p. 1-5, 2011.
Duração: 
1-5
Palavras-chaves: 
  • Trichoderma harzianum PAMB-86
  • β-(1→3)- Glucanases
  • Response surface method
  • Paramylon
  • Lichen Pustulan
  • Gluco-oligosaccharides
Fonte: 
http://omicsgroup.org/journals/production-of-glucanases-by-trichoderma-harzianum-rifai-optimization-and-application-to-produce-glucooligosaccharides-from-paramylon-and-pustulan-2167-7972.1000102.php?aid=4863
Endereço permanente: 
Direitos de acesso: 
Acesso aberto
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/122593
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