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- Biomodulation of inflammatory cytokines related to oral mucositis by low-level laser therapy
- Universidade Estadual Paulista (UNESP)
- Universidade Federal de São Carlos (UFSCAR)
- This study evaluated the effects of LLLT on the expressionof inﬂammatory cytokines related to the development of oralmucositis by gingival ﬁbroblasts. Primary gingival ﬁbroblastswere seeded on 24-well plates (105cells/well) for 24 h. Freshserum-free culture medium (DMEM) was then added, andcells were placed in contact with LPS (Escherichia coli,1 lgmL1), followed by LLLT irradiation (LaserTABLE—InGaAsP diode prototype—780 nm, 25 mW) delivering 0,0.5, 1.5 or 3 J cm². Cells without contact with LPS werealso irradiated with the same energy densities. Gene expres-sion of TNF- a, IL-1b, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines wasdetermined by enzyme-linked immunosorbent (ELISA) assay.Data were statistically analyzed by the Kruskal– Wallis test,complemented by the Mann–Whitney test (P < 0.05). LPStreatment increased the gene expression and protein synthesisof TNF-a, IL-6 and IL-8, while the expression of IL-1b wasnot affected. For LPS-treated groups, LLLT promoted signif-icant decreases in the expression of TNF-a, IL-6, and IL-8 at1.5 J cm2and 3 J cm2. These results demonstrate thatLLLT promoted a beneﬁcial biomodulatory effect on theexpression of inﬂammatory cytokines related to oral mucosi-tis by human gingival ﬁbroblasts.
- Photochemistry and Photobiology, v. 91, n. 4, p. 952-956, 2015.
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