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dc.contributor.authorGuimaraes, APA-
dc.contributor.authorDias, F. L.-
dc.contributor.authorCardoso, R. S.-
dc.contributor.authorKronka, S. N.-
dc.contributor.authorSakamoto-Hojo, E. T.-
dc.identifier.citationTeratogenesis Carcinogenesis and Mutagenesis. New York: Wiley-liss, p. 171-186, 2003.-
dc.description.abstractA cytogenetic study was carried out with 5-azacytidine (5-azaC) and etoposide (VP-16) in CHO-K1 and XRS-5 (mutant cells deficient for double-strand break rejoining) cell lines to verify the interaction effects of the drugs in terms of induction of chromosomal aberrations. 5-azaC is incorporated into DNA causing DNA hypomethylation, and VP-16 (inhibitor of topoisomerase 11 enzyme) is a potent clastogenic agent. Cells in exponential growth were treated with 5-azaC for I h, following incubation for 7 h, and posttreatment with VP16 for the last 3 h. In K1 cells, the combined treatments induced a significant reduction in the aberrations induced in the X and A (autosome) chromosomes, which are the main target for 5-azaC. However, in XRS-5 cells, the drug combination caused a significant increase in the aberrations induced in those chromosomes, but with a concomitant reduction in the randomly induced-aberrations. In addition, each cell line presented characteristic cell cycle kinetics; while the combined treatment induced an S-arrest in K1 cells, alterations in cell cycle progression were not found for XRS-5, although each drug alone caused a G2-arrest. The different cell responses presented by the cell lines may be explained on the basis of the evidence that alterations in chromatin structure caused by 5-aza-C probably occur to a different extent in K1 and XRS-5 cells, since the mutant cells present a typical hyper-condensed chromosome structure (especially the X- and A chromosomes), but, alternatively, 5-aza-C could induce reactivation of DNA repair genes in XRS-5 cells. Teratogenesis Carcinog. Mutagen. Suppl. 1:171-186, 2003. (C) 2003 Wiley-Liss, Inc.en
dc.sourceWeb of Science-
dc.subjectchromosomal aberrationspt
dc.subjectDNA methylationpt
dc.titleChromosomal aberrations induced by 5-azacytidine combined with VP-16 (etoposide) in CHO-K1 and XRS-5 cell linesen
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciências & Letras Ribeirao Pret, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil-
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Genet, São Paulo, SP, Brazil-
dc.description.affiliationUNESP, Fac Ciências Agr & Med Vet, Dept Ciências Exatas, Jaboticabal, SP, Brazil-
dc.description.affiliationUnespUNESP, Fac Ciências Agr & Med Vet, Dept Ciências Exatas, Jaboticabal, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofTeratogenesis Carcinogenesis and Mutagenesis-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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