Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/125762
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zago, Chaiene Evelin | - |
dc.contributor.author | Silva, Sónia | - |
dc.contributor.author | Sanitá, Paula Volpato | - |
dc.contributor.author | Barbugli, Paula Aboud | - |
dc.contributor.author | Dias, Carla Maria Improta | - |
dc.contributor.author | Lordello, VirgÍnia Barreto | - |
dc.contributor.author | Vergani, Carlos Eduardo | - |
dc.date.accessioned | 2015-08-06T16:13:03Z | - |
dc.date.accessioned | 2016-10-25T20:53:38Z | - |
dc.date.available | 2015-08-06T16:13:03Z | - |
dc.date.available | 2016-10-25T20:53:38Z | - |
dc.date.issued | 2015 | - |
dc.identifier | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123206 | - |
dc.identifier.citation | Plos One, v. 10, n. 4, p. e0123206, 2015. | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/11449/125762 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/125762 | - |
dc.description.abstract | Polymicrobial biofilms are an understudied and a clinically relevant problem. This study evaluates the interaction between C. albicans, and methicillin- susceptible (MSSA) and resistant (MRSA) S. aureus growing in single- and dual-species biofilms. Single and dual species adhesion (90 min) and biofilms (12, 24, and 48 h) were evaluated by complementary methods: counting colony-forming units (CFU mL-1), XTT-reduction, and crystal violet staining (CV). The secretion of hydrolytic enzymes by the 48 h biofilms was also evaluated using fluorimetric kits. Scanning electron microscopy (SEM) was used to assess biofilm structure. The results from quantification assays were compared using two-way ANOVAs with Tukey post-hoc tests, while data from enzymatic activities were analyzed by one-way Welch-ANOVA followed by Games-Howell post hoc test (α = 0.05). C. albicans, MSSA and MRSA were able to adhere and to form biofilm in both single or mixed cultures. In general, all microorganisms in both growth conditions showed a gradual increase in the number of cells and metabolic activity over time, reaching peak values between 12 h and 48 h (ρ<0.05). C. albicans single- and dual-biofilms had significantly higher total biomass values (ρ<0.05) than single biofilms of bacteria. Except for single MRSA biofilms, all microorganisms in both growth conditions secreted proteinase and phospholipase-C. SEM images revealed extensive adherence of bacteria to hyphal elements of C. albicans. C. albicans, MSSA, and MRSA can co-exist in biofilms without antagonism and in an apparent synergistic effect, with bacteria cells preferentially associated to C. albicans hyphal forms. | en |
dc.description.sponsorship | Coordenação de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | - |
dc.format.extent | e0123206 | - |
dc.language.iso | eng | - |
dc.source | Currículo Lattes | - |
dc.title | Dynamics of biofilm formation and the interaction between candida albicans and methicillin-susceptible (MSSA) and -resistant staphylococcus aureus (MRSA) | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | University of Minho | - |
dc.description.affiliation | Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Materiais Odontológicos e Prótese, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá Nº1680, Centro, CEP 14801-903, SP, Brasil | - |
dc.description.affiliationUnesp | Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Materiais Odontológicos e Prótese, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá Nº1680, Centro, CEP 14801-903, SP, Brasil | - |
dc.description.sponsorshipId | CNPq: 400658/2012-7 | - |
dc.description.sponsorshipId | FCT: SFRH/BPD/71076/2010 | - |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0123206 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | ISSN1932-6203-2015-10-04-e0123206.pdf | - |
dc.relation.ispartof | Plos One | - |
dc.identifier.lattes | 7528118481872587 | - |
dc.identifier.lattes | 3003130522427820 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.