You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/128305
Full metadata record
DC FieldValueLanguage
dc.contributor.authorReichel, Jonathan-
dc.contributor.authorChadburn, Amy-
dc.contributor.authorRubinstein, Paul G.-
dc.contributor.authorGiulino-Roth, Lisa-
dc.contributor.authorTam, Wayne-
dc.contributor.authorLiu, Yifang-
dc.contributor.authorGaiolla, Rafael-
dc.contributor.authorEng, Kenneth-
dc.contributor.authorBrody, Joshua-
dc.contributor.authorInghirami, Giorgio-
dc.contributor.authorCarlo-Stella, Carmelo-
dc.contributor.authorSantoro, Armando-
dc.contributor.authorRahal, Daoud-
dc.contributor.authorTotonchy, Jennifer-
dc.contributor.authorElemento, Olivier-
dc.contributor.authorCesarman, Ethel-
dc.contributor.authorRoshal, Mikhail-
dc.date.accessioned2015-10-21T13:08:51Z-
dc.date.accessioned2016-10-25T20:59:17Z-
dc.date.available2015-10-21T13:08:51Z-
dc.date.available2016-10-25T20:59:17Z-
dc.date.issued2015-02-12-
dc.identifierhttp://www.bloodjournal.org/content/125/7/1061?sso-checked=true-
dc.identifier.citationBlood. Washington: Amer Soc Hematology, v. 125, n. 7, p. 1061-1072, 2015.-
dc.identifier.issn0006-4971-
dc.identifier.urihttp://hdl.handle.net/11449/128305-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/128305-
dc.description.abstractClassical Hodgkin lymphoma (cHL) is characterized by sparsely distributed Hodgkin and Reed-Sternberg (HRS) cells amid reactive host background, complicating the acquisition of neoplastic DNA without extensive background contamination. We overcame this limitation by using flow-sorted HRS and intratumor T cells and optimized low-input exome sequencing of 10 patient samples to reveal alterations in genes involved in antigen presentation, chromosome integrity, transcriptional regulation, and ubiquitination. beta-2-microglobulin (B2M) is the most commonly altered gene in HRS cells, with 7 of 10 cases having inactivating mutations that lead to loss of major histocompatibility complex class I (MHC-I) expression. Enforced wild-type B2M expression in a cHL cell line restored MHC-I expression. In an extended cohort of 145 patients, the absence of B2M protein in the HRS cells was associated with lower stage of disease, younger age at diagnosis, and better overall and progression-free survival. B2M-deficient cases encompassed most of the nodular sclerosis subtype cases and only a minority of mixed cellularity cases, suggesting that B2M deficiency determines the tumor microenvironment and may define a major subset of cHL that has more uniform clinical and morphologic features. In addition, we report previously unknown genetic alterations that may render selected patients sensitive to specific targeted therapies.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.format.extent1061-1072-
dc.language.isoeng-
dc.publisherAmer Soc Hematology-
dc.sourceWeb of Science-
dc.titleFlow sorting and exome sequencing reveal the oncogenome of primary Hodgkin and Reed-Sternberg cellsen
dc.typeoutro-
dc.contributor.institutionWeill Cornell Medical College-
dc.contributor.institutionTri-Institutional Training Program in Computational Biology and Medicine-
dc.contributor.institutionNorthwestern University-
dc.contributor.institutionRush University Medical Center-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionIcahn School of Medicine at Mount Sinai-
dc.contributor.institutionHumanitas Clinical and Research Center-
dc.contributor.institutionUniversity of Milan-
dc.description.affiliationWeill Cornell Medical College, Department of Pathology and Laboratory Medicine-
dc.description.affiliationNorthwestern University, Department of Pathology, Feinberg School of Medicine-
dc.description.affiliationRush University Medical Center, John H. Stroger Jr Hospital of Cook County-
dc.description.affiliationWeill Cornell Medical College, Department of Pediatrics-
dc.description.affiliationHumanitas Clinical and Research Center, Humanitas Cancer Center-
dc.description.affiliationUniversity of Milan, School of Medicine-
dc.description.affiliationWeill Cornell Medical College, Institute for Computational Biomedicine-
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Clínica Médica, Faculdade de Medicina de Botucatu-
dc.identifier.doihttp://dx.doi.org/10.1182/blood-2014-11-610436-
dc.identifier.wosWOS:000350818800007-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBlood-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.