You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/128621
Full metadata record
DC FieldValueLanguage
dc.contributor.authorAraya-Jaime, Cristian-
dc.contributor.authorSerrano, Erica Alves-
dc.contributor.authorZerbinato de Andrade Silva, Duilio Mazzoni-
dc.contributor.authorYamashita, Masakane-
dc.contributor.authorIwai, Toshiharu-
dc.contributor.authorOliveira, Claudio-
dc.contributor.authorForesti, Fausto-
dc.date.accessioned2015-10-21T13:11:35Z-
dc.date.accessioned2016-10-25T21:00:03Z-
dc.date.available2015-10-21T13:11:35Z-
dc.date.available2016-10-25T21:00:03Z-
dc.date.issued2015-01-27-
dc.identifierhttp://link.springer.com/article/10.1186%2Fs13039-015-0108-9#page-1-
dc.identifier.citationMolecular Cytogenetics, v. 8, 6 p., 2015.-
dc.identifier.issn1755-8166-
dc.identifier.urihttp://hdl.handle.net/11449/128621-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/128621-
dc.description.abstractBackground: Different moderrn methodologies are presently available to analyze meiotic chromosomes. These methods permit investigation of the behavior of chromosomes in the normal complement and of sex and B chromosomes, two special types of chromosomes that are associated with the A complement and are present in many organisms, including fishes. However, meiotic studies are still scarce in fishes, considering the wide number of species in this group.. Here, we describe a new protocol for the visualization of the synaptonemal complex in spermatocytes and oocytes of fishes and to the sequential use of the technique with other procedures and techniques such as immunodetection of the synaptonemal complex protein with a specific antibody and co-detection of DNA sequences by FISH.Results: The meiotic surface-spreading protocol used in the present proposal worked well in representative species of four fish orders and was useful in obtaining good results even in small specimens. Fish-specific antibodies and commercial products worked similarly well to detect synaptonemal complex (SC) proteins. The sequential application of fluorescence in situ hybridization using specific probes showed clear signals associated with the SC structures identified by immunostaining.Conclusion: Here, we provide a useful and applicable immunofluorescent protocol for the visualization of synaptonemal complex proteins in the meiotic cells of fishes in surface-spreading preparations. Furthermore, this technique allows for the sequential application of other cytogenetic procedures.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.format.extent6-
dc.language.isoeng-
dc.publisherBiomed Central Ltd-
dc.sourceWeb of Science-
dc.subjectMeiosisen
dc.subjectSYCP3en
dc.subjectMethoden
dc.subjectCo-detectionen
dc.subjectFISHen
dc.titleSurface-spreading technique of meiotic cells and immunodetection of synaptonemal complex proteins in teleostean fishesen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionHokkaido Univ-
dc.contributor.institutionEhime Univ-
dc.description.affiliationUniv Estadual Paulista, Dept Morfol, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil-
dc.description.affiliationHokkaido Univ, Dept Biol Sci, Fac Sci, Sapporo, Hokkaido 0600810, Japan-
dc.description.affiliationEhime Univ, South Ehime Fisheries Res Ctr, Matsuyama, Ehime 7984292, Japan-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Morfol, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil-
dc.identifier.doihttp://dx.doi.org/10.1186/s13039-015-0108-9-
dc.identifier.wosWOS:000349120700001-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileWOS000349120700001.pdf-
dc.relation.ispartofMolecular Cytogenetics-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.