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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/128697
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dc.contributor.authorChristofoletti Mazzeo, Dania Elisa-
dc.contributor.authorMarin-Morales, Maria Aparecida-
dc.date.accessioned2015-10-21T13:12:24Z-
dc.date.accessioned2016-10-25T21:00:14Z-
dc.date.available2015-10-21T13:12:24Z-
dc.date.available2016-10-25T21:00:14Z-
dc.date.issued2015-07-01-
dc.identifierhttp://link.springer.com/article/10.1007%2Fs11356-015-4134-2-
dc.identifier.citationEnvironmental Science And Pollution Research. Heidelberg: Springer Heidelberg, v. 22, n. 13, p. 9796-9806, 2015.-
dc.identifier.issn0944-1344-
dc.identifier.urihttp://hdl.handle.net/11449/128697-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/128697-
dc.description.abstractNucleolar alterations resulting from the action of either chemical or physical agents can serve as important genotoxicity biomarkers. In this study, the efficiency of AgNOR banding technique to identify the presence of nucleoli inmicronucleus and assess nucleolar alterations in aberrant cells of Allium cepa was evaluated. Seeds of this plant were exposed to both water samples from a river that receives untreated urban effluent and to the trifluralin herbicide (0.84 mg/L concentration), both analyzed in two different seasons (summer and winter seasons). Samples induced significant frequencies of chromosomal and nuclear aberrations and micronuclei, as observed in cells submitted to conventional chromosomal staining. The herbicide caused a significant increase in the number of nucleoli and micronuclei, interpreted as due to the elimination of excessive nucleolar material resulting from polyploidization. The use of the AgNOR technique enabled the identification of both the presence of the nucleolus in some micronuclei and the nucleolar organizer region (NOR) behavior of aberrant cells. The NOR-banding technique showed to be an efficient tool for studying the genotoxic effects caused by a xenobiotics and a complex environmental sample.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.format.extent9796-9806-
dc.language.isoeng-
dc.publisherSpringer-
dc.sourceWeb of Science-
dc.subjectNucleolusen
dc.subjectAllium cepaen
dc.subjectMicronucleusen
dc.subjectUrban effluenten
dc.subjectTrifluralin herbicideen
dc.subjectAgNOR stainingen
dc.titleGenotoxicity evaluation of environmental pollutants using analysis of nucleolar alterationsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Biology, Institute of Biosciences, UNESP — Univ Estadual Paulista, Av. 24-A, 1515, 13506-900, Rio Claro, SP, Brazil-
dc.description.affiliationUnespDepartment of Biology, Institute of Biosciences, UNESP — Univ Estadual Paulista, Av. 24-A, 1515, 13506-900, Rio Claro, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 2005/01369-1-
dc.identifier.doihttp://dx.doi.org/10.1007/s11356-015-4134-2-
dc.identifier.wosWOS:000356937900023-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofEnvironmental Science And Pollution Research-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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