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DC Field | Value | Language |
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dc.contributor.author | Lindae, Antje | - |
dc.contributor.author | Eberle, Raphael J. | - |
dc.contributor.author | Caruso, Icaro P. | - |
dc.contributor.author | Coronado, Monika A. | - |
dc.contributor.author | Moraes, Fabio R. de | - |
dc.contributor.author | Azevedo, Vasco | - |
dc.contributor.author | Arni, Raghuvir K. | - |
dc.date.accessioned | 2015-10-21T13:14:22Z | - |
dc.date.accessioned | 2016-10-25T21:00:35Z | - |
dc.date.available | 2015-10-21T13:14:22Z | - |
dc.date.available | 2016-10-25T21:00:35Z | - |
dc.date.issued | 2015-08-01 | - |
dc.identifier | http://www.sciencedirect.com/science/article/pii/S1046592815000868 | - |
dc.identifier.citation | Protein Expression And Purification. San Diego: Academic Press Inc Elsevier Science, v. 112, p. 15-20, 2015. | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | http://hdl.handle.net/11449/128845 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/128845 | - |
dc.description.abstract | The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable economical loss. Cold shock proteins are conserved in many bacteria and eukaryotic cells and they help to restore normal cell functions after cold shock in which some appear to have specific functions at normal growth temperature as well.Cold shock protein A from C pseudotuberculosis was expressed in Escherichia coli and purified. The thermal unfolding/refolding process characterized by circular dichroism, differential scanning calorimetry and NMR spectroscopy techniques indicated that the refolding process was almost completely reversible. (C) 2015 Elsevier Inc. All rights reserved. | en |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
dc.format.extent | 15-20 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | C. pseudotuberculosis | en |
dc.subject | Cold shock protein | en |
dc.subject | Spectroscopic methods | en |
dc.subject | Secondary structure | en |
dc.subject | Folding and refolding proteins | en |
dc.title | Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade Federal de Minas Gerais (UFMG) | - |
dc.description.affiliation | Univ Estadual Paulista UNESP, Ctr Multiusuario Inovacao Biomol, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | - |
dc.description.affiliation | Univ Fed Minas Gerais, Lab Genet Celular &Mol, Inst Ciencias Biol, BR-270901 Belo Horizonte, MG, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista UNESP, Ctr Multiusuario Inovacao Biomol, Departamento de Física, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | - |
dc.identifier.doi | http://dx.doi.org/10.1016/j.pep.2015.04.006 | - |
dc.identifier.wos | WOS:000355572200003 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Protein Expression And Purification | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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