You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCorrea de Souza, Rafael Soares-
dc.contributor.authorBalbuena, Tiago Santana-
dc.contributor.authorArruda, Paulo-
dc.identifier.citationPlant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015.-
dc.description.abstractProlamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.publisherCrop Science Soc Amer-
dc.sourceWeb of Science-
dc.titleStructure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grassesen
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniversidade Estadual de Campinas (UNICAMP), Departamento de Genética e Evolução, Instituto de Biologia, 13083-875, Campinas, SP, Brazil-
dc.description.affiliationUniversidade Estadual Paulista (UNESP), Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, 14884-900, Jaboticabal, SP, Brazil-
dc.description.affiliationCentro de Biologia Molecular e Engenharia Genética-
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP), Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, 14884-900, Jaboticabal, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 2011/12431-0-
dc.description.sponsorshipIdFAPESP: 2011/11650-0-
dc.rights.accessRightsAcesso aberto-
dc.relation.ispartofPlant Genome-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.