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DC Field | Value | Language |
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dc.contributor.author | Rosa, Fabiola E. | - |
dc.contributor.author | Silveira, Sara M. | - |
dc.contributor.author | Silveira, Cassia G. T. | - |
dc.contributor.author | Bergamo, Nadia A. | - |
dc.contributor.author | Moraes Neto, Francisco A. | - |
dc.contributor.author | Domingues, Maria Aparecida Custódio | - |
dc.contributor.author | Soares, Fernando A. | - |
dc.contributor.author | Caldeira, Jose R. F. | - |
dc.contributor.author | Rogatto, Silvia Regina | - |
dc.date.accessioned | 2014-05-20T13:37:22Z | - |
dc.date.available | 2014-05-20T13:37:22Z | - |
dc.date.issued | 2009-03-23 | - |
dc.identifier | http://dx.doi.org/10.1186/1471-2407-9-90 | - |
dc.identifier.citation | Bmc Cancer. London: Biomed Central Ltd., v. 9, p. 12, 2009. | - |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | http://hdl.handle.net/11449/12920 | - |
dc.description.abstract | Background: HER-2 gene testing has become an integral part of breast cancer patient diagnosis. The most commonly used assay in the clinical setting for evaluating HER-2 status is immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). These procedures permit correlation between HER-2 expression and morphological features. However, FISH signals are labile and fade over time, making post-revision of the tumor difficult. CISH (chromogenic in situ hybridization) is an alternative procedure, with certain advantages, although still limited as a diagnostic tool in breast carcinomas.Methods: To elucidate the molecular profile of HER-2 status, mRNA and protein expression in 75 invasive breast carcinomas were analyzed by real time quantitative RT-PCR (qRT-PCR) and IHC, respectively. Amplifications were evaluated in 43 of these cases by CISH and in 11 by FISH.Results: The concordance rate between IHC and qRT-PCR results was 78.9%, and 94.6% for qRT-PCR and CISH. Intratumoral heterogeneity of HER-2 status was identified in three cases by CISH. The results of the three procedures were compared and showed a concordance rate of 83.8%; higher discordances were observed in 0 or 1+immunostaining cases, which showed high-level amplification (15.4%) and HER-2 transcript overexpression (20%). Moreover, 2+ immunostaining cases presented nonamplified status (50%) by CISH and HER-2 downexpression (38.5%) by qRT-PCR. In general, concordance occurred between qRT-PCR and CISH results. A high concordance was observed between CISH/qRT-PCR and FISH. Comparisons with clinicopathological data revealed a significant association between HER-2 downexpression and the involvement of less than four lymph nodes (P = 0.0350).Conclusion: Based on these findings, qRT-PCR was more precise and reproducible than IHC. Furthermore, CISH was revealed as an alternative and useful procedure for investigating amplifications involving the HER-2 gene. | en |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.format.extent | 12 | - |
dc.language.iso | eng | - |
dc.publisher | Biomed Central Ltd. | - |
dc.source | Web of Science | - |
dc.title | Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Amaral Carvalho Hosp | - |
dc.contributor.institution | Hosp AC Camargo Fund Antonio Prudente | - |
dc.contributor.institution | Hosp AC Camargo Liberdade São Paulo | - |
dc.description.affiliation | UNESP São Paulo State Univ, Fac Med, Dept Urol, Botucatu, SP, Brazil | - |
dc.description.affiliation | UNESP São Paulo State Univ, Inst Biosci, Dept Genet, Botucatu, SP, Brazil | - |
dc.description.affiliation | Amaral Carvalho Hosp, Dept Pathol, São Paulo, Brazil | - |
dc.description.affiliation | UNESP São Paulo State Univ, Fac Med, Dept Pathol, Botucatu, SP, Brazil | - |
dc.description.affiliation | Hosp AC Camargo Fund Antonio Prudente, Fundação Antonio Prudente, Dept Pathol, São Paulo, Brazil | - |
dc.description.affiliation | Amaral Carvalho Hosp, Dept Senol, São Paulo, Brazil | - |
dc.description.affiliation | Hosp AC Camargo Liberdade São Paulo, Fundação Antonio Prudente 211, NeoGene Lab, São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP São Paulo State Univ, Fac Med, Dept Urol, Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP São Paulo State Univ, Inst Biosci, Dept Genet, Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP São Paulo State Univ, Fac Med, Dept Pathol, Botucatu, SP, Brazil | - |
dc.identifier.doi | 10.1186/1471-2407-9-90 | - |
dc.identifier.wos | WOS:000265611700001 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | WOS000265611700001.pdf | - |
dc.relation.ispartof | BMC Cancer | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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