Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/129787
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Silva Junior, Willer Ferreira da | - |
dc.contributor.author | Cano, Rodrigo | - |
dc.contributor.author | Totola, Antonio Helvecio | - |
dc.contributor.author | Carvalho, Lorendane Milena de | - |
dc.contributor.author | Cerri, Marcel Otavio | - |
dc.contributor.author | Reis Coimbra, Jane Sella dos | - |
dc.contributor.author | Pinto de Carvalho, Gleidson Giordano | - |
dc.contributor.author | Aparecida de Carvalho, Bruna Mara | - |
dc.date.accessioned | 2015-10-22T07:08:08Z | - |
dc.date.accessioned | 2016-10-25T21:16:29Z | - |
dc.date.available | 2015-10-22T07:08:08Z | - |
dc.date.available | 2016-10-25T21:16:29Z | - |
dc.date.issued | 2015-05-22 | - |
dc.identifier | http://www.sciencedirect.com/science/article/pii/S0021967315004628 | - |
dc.identifier.citation | Journal Of Chromatography A. Amsterdam: Elsevier Science Bv, v. 1395, p. 16-22, 2015. | - |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | http://hdl.handle.net/11449/129787 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/129787 | - |
dc.description.abstract | The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu2+ cryogel system was studied by batch equilibrium Measurements. The adsorptive equilibrium data were obtained at 17 and 27 degrees C, pH 5.0 and 6.5, and at ionic strength of 50 and 200 mmol L-1 NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu2+ cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27 degrees C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27 mg g(-1). The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes. (C) 2015 Elsevier BY. All rights reserved. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.format.extent | 16-22 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Immunoglobulin Y | en |
dc.subject | Adsorption isotherms | en |
dc.subject | Thermodynamic parameters | en |
dc.subject | Cryogel | en |
dc.subject | IMAC | en |
dc.title | Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions | en |
dc.type | outro | - |
dc.contributor.institution | Univ Fed Sao Joao del Rei | - |
dc.contributor.institution | Universidade Federal de Viçosa (UFV) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade Federal da Bahia (UFBA) | - |
dc.contributor.institution | Universidade Federal de Minas Gerais (UFMG) | - |
dc.description.affiliation | Univ Fed Sao Joao del Rei, Dept Chem Biotechnol &Bioproc Engn, BR-36420000 Ouro Branco, MG, Brazil | - |
dc.description.affiliation | Univ Fed Vicosa, Dept Vet, BR-36570000 Vicosa, MG, Brazil | - |
dc.description.affiliation | Sao Paulo State Univ, Dept Bioproc &Biotechnol, BR-14801902 Araraquara, SP, Brazil | - |
dc.description.affiliation | Univ Fed Vicosa, Dept Food Technol, BR-36570000 Vicosa, MG, Brazil | - |
dc.description.affiliation | Univ Fed Bahia, Dept Anim Sci, BR-40110909 Salvador, BA, Brazil | - |
dc.description.affiliation | Univ Fed Minas Gerais, Inst Agr Sci, BR-39400401 Montes Claros, MG, Brazil | - |
dc.description.affiliationUnesp | Sao Paulo State Univ, Dept Bioproc &Biotechnol, BR-14801902 Araraquara, SP, Brazil | - |
dc.identifier.doi | http://dx.doi.org/10.1016/j.chroma.2015.03.052 | - |
dc.identifier.wos | WOS:000354143100003 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Journal Of Chromatography A | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.