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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/130709
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dc.contributor.authorMendes, Maria Anita-
dc.contributor.authorSouza, Bibiana Monson de-
dc.contributor.authorSantos, Lucilene Delazari dos-
dc.contributor.authorPalma, Mario Sergio-
dc.date.accessioned2014-05-27T11:21:04Z-
dc.date.accessioned2016-10-25T21:21:50Z-
dc.date.available2014-05-27T11:21:04Z-
dc.date.available2016-10-25T21:21:50Z-
dc.date.issued2004-04-15-
dc.identifierhttp://dx.doi.org/10.1002/rcm.1382-
dc.identifier.citationRapid Communications in Mass Spectrometry, v. 18, n. 6, p. 636-642, 2004.-
dc.identifier.issn0951-4198-
dc.identifier.urihttp://hdl.handle.net/11449/130709-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/130709-
dc.description.abstractHigh-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) techniques were applied for the detection, purification, monitoring, and sequencing of two novel and biologically active peptides occurring at very low levels in the venom of the wasp Agelaia pallipes pallipes. These peptides were sequenced under LC/ESI-MS/MS conditions and designated as Agelaia-CP (I/L-L-G-T-I-L-G-L-L-K-G-I/L-NH2, MW 1207.8Da) and Agelaia-MP (I/L-N-W-L-K-L-G-K-A-I-I-D-A-I/L-NH2, MW 1565.0Da). The peptide Agelaia-CP showed no hemolytic activity, but it behaved as a mast cell degranulator and induced a potent chemotaxis in polymorphonucleated leukocyte (PMNL) cells, typical of a wasp chemotactic peptide. The peptide Agelaia-MP showed both powerful mast cell degranulation and hemolysis of washed rat red blood cells, and is thus assigned as a new member of the mastoparan family of peptides. Both peptides seem to be directly involved in the strong inflammatory reactions associated with wasp stings. © Copyright 2004 John Wiley & Sons, Ltd.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent636-642-
dc.language.isoeng-
dc.publisherWiley-Blackwell-
dc.sourceScopus-
dc.subjectChemotactic peptide-
dc.subjectMastoparan-
dc.subjectUnclassified drug-
dc.subjectWasp venom-
dc.subjectAgelaia pallipes pallipes-
dc.subjectAnimal cell-
dc.subjectBee sting-
dc.subjectElectrospray mass spectrometry-
dc.subjectHemolysis-
dc.subjectHigh performance liquid chromatography-
dc.subjectInflammation-
dc.subjectMast cell degranulation-
dc.subjectNeutrophil chemotaxis-
dc.subjectNonhuman-
dc.subjectProtein analysis-
dc.subjectPprotein purification-
dc.subjectProtein structure-
dc.subjectRat-
dc.subjectSequence analysis-
dc.subjectTandem mass spectrometry-
dc.subjectWasp-
dc.subjectWasp sting-
dc.subjectAnimals-
dc.subjectChemotactic Factors-
dc.subjectChromatography, High Pressure Liquid-
dc.subjectMolecular Structure-
dc.subjectPeptides-
dc.subjectSequence Analysis, Protein-
dc.subjectSpectrometry, Mass, Electrospray Ionization-
dc.subjectWasp Venoms-
dc.subjectWasps-
dc.titleStructural characterization of novel chemotactic and mastoparan peptides from the venom of the social wasp Agelaia pallipes pallipes by high-performance liquid chromatography/electrospray ionization tandem mass spectrometryen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationCEIS - Dept. of Biology/IBRC-UNESP Inst. of Immunol. Investigations Millennium Institute-MCT/CNPq, Rio Claro/SP 13506-900-
dc.description.affiliationUnespCEIS - Dept. of Biology/IBRC-UNESP Inst. of Immunol. Investigations Millennium Institute-MCT/CNPq, Rio Claro/SP 13506-900-
dc.identifier.doi10.1002/rcm.1382-
dc.identifier.wosWOS:000220467000006-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofRapid Communications in Mass Spectrometry-
dc.identifier.scopus2-s2.0-1842585620-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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