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DC Field | Value | Language |
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dc.contributor.author | Frión-Herrera, Yahima | - |
dc.contributor.author | Díaz-García, Alexis | - |
dc.contributor.author | Ruiz-Fuentes, Jenny | - |
dc.contributor.author | Rodríguez-Sánchez, Hermis | - |
dc.contributor.author | Sforcin, José Maurício | - |
dc.date.accessioned | 2015-12-07T15:32:53Z | - |
dc.date.accessioned | 2016-10-25T21:23:02Z | - |
dc.date.available | 2015-12-07T15:32:53Z | - |
dc.date.available | 2016-10-25T21:23:02Z | - |
dc.date.issued | 2015 | - |
dc.identifier | http://dx.doi.org/10.1111/jphp.12449 | - |
dc.identifier.citation | The Journal Of Pharmacy And Pharmacology, v. 67, n. 10, p. 1448-1456, 2015. | - |
dc.identifier.issn | 2042-7158 | - |
dc.identifier.uri | http://hdl.handle.net/11449/131233 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/131233 | - |
dc.description.abstract | Propolis effect on the growth and apoptosis of human lung adenocarcinoma (A549 cells) was investigated as well as its mechanisms. Cells were incubated with propolis for 72 h, and 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were employed to assess cell viability and the inhibitory concentration (IC). Apoptosis was detected by Acridine Orange/Ethidium Bromide and 4',6-diamidino-2-phenylindole staining after 24 and 48 h of incubation with ¼ IC50 of propolis by testing the mitochondrial membrane potential (ΔΨm) and the expression of apoptosis-related genes (p53, Caspase-3, Bax, Bcl-2, Bcl-XL , Noxa, Puma and p21) by reverse transcription polymerase chain reaction. Propolis displayed antiproliferative and cytotoxic effects on A549 cells in a dose- and time-dependent manner, but it did not suppress the growth of normal Vero cells. An enhanced apoptosis was seen in A549 propolis-treated cells after 48 h compared with the control cells. Propolis decreased mitochondrial membrane potential by overexpression of pro-apoptotic genes (Bax and Noxa) and reduction of the antiapoptotic gene Bcl-XL . The expression level of other genes remained unchanged (p53, Caspse-3 and Bax), whereas p21 expression was increased. Propolis induced caspase-independent apoptosis through a p53-independent mitochondrial pathway, and cell cycle arrest by upregulation of p21. Although propolis induces apoptosis mainly by p53-independent manner, it may be induced by another pathway, and new insights may arise for preventing or treating lung cancer. | en |
dc.format.extent | 1448-1456 | - |
dc.language.iso | eng | - |
dc.source | PubMed | - |
dc.subject | Apoptosis | en |
dc.subject | Lung cancer | en |
dc.subject | Mitochondria pathway | en |
dc.subject | Propolis | en |
dc.title | Brazilian green propolis induced apoptosis in human lung cancer A549 cells through mitochondrial-mediated pathway | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Laboratories of Biofarmaceuticals and Chemistries Productions (LABIOFAM) | - |
dc.contributor.institution | Tropical Medicine Institute Pedro Kouri | - |
dc.description.affiliationUnesp | Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu | - |
dc.identifier.doi | 10.1111/jphp.12449 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | The Journal Of Pharmacy And Pharmacology | - |
dc.identifier.pubmed | 26206395 | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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