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dc.contributor.authorMorais, Isabel C. O.-
dc.contributor.authorPereira, Gustavo J. S.-
dc.contributor.authorOrzáez, M.-
dc.contributor.authorJorge, Roberta J. B.-
dc.contributor.authorBincoletto, Claudia-
dc.contributor.authorToyama, Marcos H.-
dc.contributor.authorMonteiro, Helena S. A.-
dc.contributor.authorSmaili, Soraya S.-
dc.contributor.authorPérez-Payá, Enrique-
dc.contributor.authorMartins, Alice M. C.-
dc.date.accessioned2015-12-07T15:33:43Z-
dc.date.accessioned2016-10-25T21:23:13Z-
dc.date.available2015-12-07T15:33:43Z-
dc.date.available2016-10-25T21:23:13Z-
dc.date.issued2015-
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0132569-
dc.identifier.citationPlos One, v. 10, n. 7, 2015.-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/11449/131309-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/131309-
dc.description.abstractAcute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B. leucurus venom called L-aminoacid oxidase (LAAO-Bl) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO-Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induction was accompanied by Ca2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO-Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO-Bl (10 μg/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO-Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO-Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Ceará (FUNCAP)-
dc.language.isoeng-
dc.publisherPublic Library Science-
dc.sourcePubMed-
dc.titleL-Aminoacid oxidase from bothrops leucurus venom Induces nephrotoxicity via apoptosis and necrosisen
dc.typeoutro-
dc.contributor.institutionUniversidade Federal do Ceará (UFC)-
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionCentro de Investigación Príncipe Felipe-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil-
dc.description.affiliationDepartment of Pharmacology, Federal University of São Paulo (UNIFESP), São Paulo, Brazil-
dc.description.affiliationDepartment of Medicinal Chemistry, Centro de Investigación Príncipe Felipe, Valencia, Spain-
dc.description.affiliationSão Vicente Unit, Paulista Coastal Campus, São Paulo State University (UNESP), São Paulo, Brazil-
dc.description.affiliationDepartment of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Ceará, Brazil-
dc.description.affiliationUnespSão Vicente Unit, Paulista Coastal Campus, São Paulo State University (UNESP), São Paulo, Brazil.-
dc.description.sponsorshipIdFAPESP: 2008/11515-3-
dc.description.sponsorshipIdFAPESP: 2010/51647-6-
dc.identifier.doi10.1371/journal.pone.0132569-
dc.rights.accessRightsAcesso aberto-
dc.identifier.filePMC4508040.pdf-
dc.relation.ispartofPlos One-
dc.identifier.pubmed26193352-
dc.identifier.pmcPMC4508040-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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