You are in the accessibility menu

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/131320
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRavalli, Andrea-
dc.contributor.authorRocha, Carolina Gomes da-
dc.contributor.authorYamanaka, Hideko-
dc.contributor.authorMarrazza, Giovanna-
dc.date.accessioned2015-12-07T15:33:50Z-
dc.date.accessioned2016-10-25T21:23:15Z-
dc.date.available2015-12-07T15:33:50Z-
dc.date.available2016-10-25T21:23:15Z-
dc.date.issued2015-
dc.identifierhttp://dx.doi.org/10.1016/j.bioelechem.2015.07.010-
dc.identifier.citationBioelectrochemistry (amsterdam, Netherlands), v. 106, parte B, p. 268-275, 2015.-
dc.identifier.issn1878-562X-
dc.identifier.urihttp://hdl.handle.net/11449/131320-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/131320-
dc.description.abstractIn this paper, we report the development of a sensitive label-free impedimetric biosensor based on the use of affibody as bioreceptor and gold nanostructured screen-printed graphite as a sensor platform for the detection of human epidermal growth factor receptor 2 (HER2). The affisensor is realized by immobilizing a terminal cysteine-modified affibody on gold nanoparticles. The sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Furthermore, surface plasmon resonance (SPR) technology was also applied to explore the potential of affibodies as small-molecule discriminating tools. Using optimized experimental conditions, a single-use affisensor showed a good analytical performance for HER2 detection from 0 to 40μg/L. The estimated limit of detection was 6.0μg/L. Finally, the realized affisensor was applied to human serum samples.en
dc.format.extent268-275-
dc.language.isoeng-
dc.publisherElsevier B. V.-
dc.sourcePubMed-
dc.subjectAffibodyen
dc.subjectBiosensoren
dc.subjectHer2en
dc.subjectNanoparticleen
dc.subjectTumor markeren
dc.titleA label-free electrochemical affisensor for cancer marker detection: the case of HER2en
dc.typeoutro-
dc.contributor.institutionUniversity of Florence-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Chemistry Ugo Schiff, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy. Electronic address: andrea.ravalli@unifi.it.-
dc.description.affiliationDepartment of Chemistry Ugo Schiff, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy; Department of Analytical Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: carolgomesrocha@hotmail.com.-
dc.description.affiliationDepartment of Analytical Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil. Electronic address: hidekoy@iq.unesp.br.-
dc.description.affiliationDepartment of Chemistry Ugo Schiff, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy. Electronic address: giovanna.marrazza@unifi.it.-
dc.description.affiliationUnespDepartment of Analytical Chemistry, São Paulo State University (UNESP), Rua Prof. Francisco Degni 55, 14800-060 Araraquara, SP, Brazil-
dc.identifier.doi10.1016/j.bioelechem.2015.07.010-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBioelectrochemistry (amsterdam, Netherlands)-
dc.identifier.pubmed26260578-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.
Show simple item record Show full item record   View Statistics
 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.