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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/131545
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dc.contributor.authorColetta, Amanda Manoel Della-
dc.contributor.authorBachiega, Tatiana Fernanda-
dc.contributor.authorSilva, Juliana Carvalho de Quaglia e-
dc.contributor.authorSoares, Ângela Maria Victoriano de Campos-
dc.contributor.authorFaveri, Julio de-
dc.contributor.authorMarques, Silvio Alencar-
dc.contributor.authorMarques, Mariângela Esther Alencar-
dc.contributor.authorXimenes, Valdecir Farias-
dc.contributor.authorDias-Melicio, Luciane Alarcão-
dc.date.accessioned2015-12-07T15:37:19Z-
dc.date.accessioned2016-10-25T21:23:47Z-
dc.date.available2015-12-07T15:37:19Z-
dc.date.available2016-10-25T21:23:47Z-
dc.date.issued2015-09-01-
dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0004037-
dc.identifier.citationPlos Neglected Tropical Diseases, v. 9, n. 9, p. e0004037, 2015.-
dc.identifier.issn1935-2735-
dc.identifier.urihttp://hdl.handle.net/11449/131545-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/131545-
dc.description.abstractParacoccidioidomycosis (PCM) is a systemic mycosis, endemic in most Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus of the genus Paracoccidioides (Paracoccidioides brasiliensis and Paracoccidioides lutzii). Innate immune response plays a crucial role in host defense against fungal infections, and neutrophils (PMNs) are able to combat microorganisms with three different mechanisms: phagocytosis, secretion of granular proteins, which have antimicrobial properties, and the most recent described mechanism called NETosis. This new process is characterized by the release of net-like structures called Neutrophil Extracellular Traps (NETs), which is composed of nuclear (decondensed DNA and histones) and granular material such as elastase. Several microorganisms have the ability of inducing NETs formation, including gram-positive and gram-negative bacteria, viruses and some fungi. We proposed to identify NETs in tegumentary lesions of patients with PCM and to analyze the interaction between two strains of P. brasiliensis and human PMNs by NETs formation in vitro. In this context, the presence of NETs in vivo was evidenced in tegumentary lesions of patients with PCM by confocal spectrum analyzer. Furthermore, we showed that the high virulent P. brasiliensis strain 18 (Pb18) and the lower virulent strain Pb265 are able to induce different patterns of NETs formation in vitro. The quantification of extracellular DNA corroborates the idea of the ability of P. brasiliensis in inducing NETs release. In conclusion, our data show for the first time the identification of NETs in lesions of patients with PCM and demonstrate distinct patterns of NETs in cultures challenged with fungi in vitro. The presence of NETs components both in vivo and in vitro open new possibilities for the detailed investigation of immunity in PCM.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)-
dc.language.isoeng-
dc.publisherPublic Library Science-
dc.sourcePubMed-
dc.titleNeutrophil extracellular traps identification in tegumentary lesions of patients with paracoccidioidomycosis and different patterns of NETs generation in vitroen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationDepartment of Pathology, UNESP - São Paulo State University, Botucatu Medical School, Botucatu, São Paulo, Brazil-
dc.description.affiliationDepartment of Microbiology and Immunology, UNESP-São Paulo State University, Biosciences Institute, Botucatu, São Paulo, Brazil-
dc.description.affiliationDepartment of Dermatology and Radiotherapy, UNESP - São Paulo State University, Botucatu Medical School, Botucatu, São Paulo, Brazil-
dc.description.affiliationDepartment of Chemistry, UNESP-São Paulo State University, School of Sciences, Bauru, São Paulo, Brazil-
dc.description.affiliationUnespDepartment of Pathology, UNESP - São Paulo State University, Botucatu Medical School, Botucatu, São Paulo, Brazil-
dc.description.affiliationUnespDepartment of Microbiology and Immunology, UNESP-São Paulo State University, Biosciences Institute, Botucatu, São Paulo, Brazil-
dc.description.affiliationUnespDepartment of Dermatology and Radiotherapy, UNESP - São Paulo State University, Botucatu Medical School, Botucatu, São Paulo, Brazil-
dc.description.affiliationUnespDepartment of Chemistry, UNESP-São Paulo State University, School of Sciences, Bauru, São Paulo, Brazil-
dc.description.sponsorshipIdFAPESP: 2011/18855-7-
dc.description.sponsorshipIdCNPq: 480486/2011-5-
dc.description.sponsorshipIdFUNDUNESP: 00637/11-DFP-
dc.description.sponsorshipIdFAPESP: 2013/00788-7-
dc.identifier.doi10.1371/journal.pntd.0004037-
dc.rights.accessRightsAcesso aberto-
dc.identifier.filePMC4556621.pdf-
dc.relation.ispartofPlos Neglected Tropical Diseases-
dc.identifier.pubmed26327485-
dc.identifier.pmcPMC4556621-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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