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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/131622
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dc.contributor.authorHeckler, Rafael Pereira-
dc.contributor.authorBorges, Dyego Gonçalves Lino-
dc.contributor.authorBacha, Flávia Barbieri-
dc.contributor.authorOnizuka, Marcel Kenzo Vilalba-
dc.contributor.authorTeruya, Letícia E. Silva-
dc.contributor.authorNeves, Juliana Paniago Lordelo-
dc.contributor.authorLeal, Cássia Rejane Brito-
dc.contributor.authorLemos, Ricardo Antônio Amaral de-
dc.contributor.authorMeireles, Marcelo Vasconcelos-
dc.contributor.authorBorges, Fernando de Almeida-
dc.date.accessioned2015-12-07T15:38:53Z-
dc.date.accessioned2016-10-25T21:23:59Z-
dc.date.available2015-12-07T15:38:53Z-
dc.date.available2016-10-25T21:23:59Z-
dc.date.issued2015-10-01-
dc.identifierhttp://dx.doi.org/10.1016/j.prevetmed.2015.08.016-
dc.identifier.citationPreventive Veterinary Medicine, v. 121, n. 3-4, p. 391-394, 2015.-
dc.identifier.issn1873-1716-
dc.identifier.urihttp://hdl.handle.net/11449/131622-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/131622-
dc.description.abstractThe presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.en
dc.format.extent391-394-
dc.language.isoeng-
dc.publisherElsevier B. V.-
dc.sourcePubMed-
dc.subjectCryptosporidiosisen
dc.subjectDiarrheaen
dc.subjectIiaa14g2r1en
dc.subjectIiaa15g2r1en
dc.subjectOocystsen
dc.subjectGp60en
dc.titleFirst genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazilen
dc.typeoutro-
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil-
dc.description.affiliationFaculdade de Medicina Veterinária-Universidade Estadual Paulista-UNESP, Araçatuba, Brazil-
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, MS 79070-900, Brazil-
dc.description.affiliationUnespFaculdade de Medicina Veterinária-Universidade Estadual Paulista-UNESP, Araçatuba, Brazil.-
dc.identifier.doi10.1016/j.prevetmed.2015.08.016-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofPreventive Veterinary Medicine-
dc.identifier.orcid0000-0003-0063-5172pt
dc.identifier.pubmed26342791-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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