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dc.contributor.authorCastro, Myrella L.-
dc.contributor.authorFranco, Gilson C N-
dc.contributor.authorBranco-de-Almeida, Luciana S.-
dc.contributor.authorAnbinder, Ana L.-
dc.contributor.authorCogo-Müller, Karina-
dc.contributor.authorCortelli, Sheila C.-
dc.contributor.authorDuarte, Simone-
dc.contributor.authorSaxena, Deepak-
dc.contributor.authorRosalen, Pedro L.-
dc.identifier.citationJournal Of Periodontology, p. 1-11, 2015.-
dc.description.abstractSubantimicrobial dose doxycycline (SDD) has been used as an adjunct in periodontal treatment due to its matrix metalloproteinase inhibition properties. Although the benefits of SDD therapy, such as improvement in the parameters of periodontal probing depth and clinical attachment level, have been proven in multiple clinical studies, the comprehension of other biological mechanisms of action on periodontitis remains poorly investigated. Therefore, this animal model study evaluated the effects of SDD monotherapy on the expressions of the following key pro-inflammatory genes: Proteinase-Actived Receptor-2 (PAR2), Tumor Necrosis Factor alpha (TNF-α), Interleukin-17 (IL-17), and. IL-1β. Male Wistar rats were randomly assigned to: A) Control group: no ligature-induced periodontitis and no treatment; B) Ligature group: ligature-induced periodontitis and placebo treatment and C) Ligature + doxycycline group: ligature-induced periodontitis and SDD treatment. After the experimental time, animals were sacrificed and Reverse Transcriptase-Polymerase Chain Reaction was performed to analyze the mRNA expression of IL-1β, IL-17, TNF-α, and PAR2 in gingival tissue samples. Histological analyses were performed on the furcation region and mesial gingiva of mandibular first molars to measure periodontal bone loss and collagen content. SDD administration significantly downregulated PAR2, IL-17, TNF-α, and IL-1β mRNA expressions (p<0.05). In addition, SDD treatment was accompanied by lower rates of alveolar bone loss (p<0.05) and by maintenance of the amount of gingival collagen fibers. These findings reveal new perspectives regarding SDD efficacy since it can be partially related to pro-inflammatory gene expression modulation, even considering PAR2 and IL-17, which has not been investigated thus far.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.publisherJournal of Periodontology-
dc.subjectAlveolar bone lossen
dc.subjectPeriodontal diseasesen
dc.titleDown-regulation of protease activated receptor 2, interleukin-17 and other pro-inflammatory genes by subantimicrobial doxycycline dose in a rat periodontitis modelen
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Estadual de Ponta Grossa (UEPG)-
dc.contributor.institutionUniversidade Federal do Maranhão (UFMA)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de Santo Amaro (UNISA)-
dc.contributor.institutionUniversidade de Taubaté (UNITAU)-
dc.contributor.institutionNew York University-
dc.description.affiliationDepartment of Physiological Sciences, Piracicaba Dental School, State University of Campinas, SP, Brazil.-
dc.description.affiliationDepartment of General Biology, State University of Ponta Grossa - Ponta Grossa, PR, Brazil.-
dc.description.affiliationDepartment of DentistryIIFederal University of Maranhão, São Luís, MA, Brazil.-
dc.description.affiliationDepartment of Bioscience and Oral Diagnosis, Institute of Science and Technology-São José dos Campos, Universidade Estadual Paulista-UNESP, SP, Brazil.-
dc.description.affiliationDepartment of Dentistry, Implantology Area, University of Santo Amaro, São Paulo, São Paulo, Brazil.-
dc.description.affiliationNucleus of periodontal research, University of Taubaté, Taubaté, SP, Brazil.-
dc.description.affiliationDepartment of Basic Science and Craniofacial Biology, College of Dentistry, New York University, NY, USA.-
dc.description.affiliationUnespDepartment of Bioscience and Oral Diagnosis, Institute of Science and Technology-São José dos Campos, Universidade Estadual Paulista-UNESP, SP, Brazil.-
dc.description.sponsorshipIdCNPq: 142559/2008-3-
dc.description.sponsorshipIdCNPq: 201815/2009-5-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal Of Periodontology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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