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dc.contributor.authorUllah, Anwar-
dc.contributor.authorMariutti, Ricardo Barros-
dc.contributor.authorMasood, Rehana-
dc.contributor.authorCaruso, Icaro Putinhon-
dc.contributor.authorCosta, Gustavo Henrique-
dc.contributor.authorFreita, Cristhyane Millena de-
dc.contributor.authorSantos, Camila Ramos-
dc.contributor.authorZanphorlin, Leticia Maria-
dc.contributor.authorRossini Mutton, Márcia Justino-
dc.contributor.authorMurakami, Mario Tyago-
dc.contributor.authorArni, Raghuvir Krishnaswamy-
dc.identifier.citationBiochemical And Biophysical Research Communications, v. 468, n. 1-2, p. 365-371, 2015.-
dc.description.abstract2S albumins, the seed storage proteins, are the primary sources of carbon and nitrogen and are involved in plant defense. The mature form of Moringa oleifera (M. oleifera), a chitin binding protein isoform 3-1 (mMo-CBP3-1) a thermostable antifungal, antibacterial, flocculating 2S albumin is widely used for the treatment of water and is potentially interesting for the development of both antifungal drugs and transgenic crops. The crystal structure of mMo-CBP3-1 determined at 1.7 Å resolution demonstrated that it is comprised of two proteolytically processed α-helical chains, stabilized by four disulfide bridges that is stable, resistant to pH changes and has a melting temperature (TM) of approximately 98 °C. The surface arginines and the polyglutamine motif are the key structural factors for the observed flocculating, antibacterial and antifungal activities. This represents the first crystal structure of a 2S albumin and the model of the pro-protein indicates the structural changes that occur upon formation of mMo-CBP3-1 and determines the structural motif and charge distribution patterns for the diverse observed activities.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científco e Tecnológico (CNPq)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.publisherElsevier B. V.-
dc.subject2s albuminen
dc.subjectCrystal structureen
dc.subjectFlocculating activityen
dc.subjectMoringa oleifera seedsen
dc.titleCrystal structure of mature 2S albumin from Moringa oleifera seedsen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionLaboratório Nacional de Biociências (LNBio)-
dc.contributor.institutionLaboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE)-
dc.contributor.institutionCentro Nacional de Pesquisa em Energia e Materiais (CNPEM)-
dc.description.affiliationMultiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil-
dc.description.affiliationDepartamento de Tecnologia, FCAV/UNESP, Campus de Jaboticabal, Brazil-
dc.description.affiliationBrazilian Biosciences National Laboratory (LNBio), National Center for Research in Energy and Materials, Campinas, SP, 13083-970, Brazil-
dc.description.affiliationBrazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil.-
dc.description.affiliationUnespMultiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil.-
dc.description.affiliationUnespDepartamento de Tecnologia, FCAV/UNESP, Campus de Jaboticabal, Brazil.-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBiochemical And Biophysical Research Communications-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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