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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/131669
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dc.contributor.authorSebastião, Fernanda de A-
dc.contributor.authorLemos, Eliana G M-
dc.contributor.authorPilarski, Fabiana-
dc.date.accessioned2015-12-07T15:39:55Z-
dc.date.accessioned2016-10-25T21:24:05Z-
dc.date.available2015-12-07T15:39:55Z-
dc.date.available2016-10-25T21:24:05Z-
dc.date.issued2015-10-28-
dc.identifierhttp://dx.doi.org/10.1016/j.mimet.2015.10.021-
dc.identifier.citationJournal Of Microbiological Methods, p. 1-25, 2015.-
dc.identifier.issn1872-8359-
dc.identifier.urihttp://hdl.handle.net/11449/131669-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/131669-
dc.description.abstractStreptococcus agalactiae (GBS) are Gram-positive cocci responsible for substantial losses in tilapia fish farms in Brazil and worldwide. It causes septicemia, meningoencephalitis and mortality of whole shoals that can occur within 72h. Thus, diagnostic methods are needed that are rapid, specific and sensitive. In this study, a pair of specific primers for GBS was generated based on the cfb gene sequence and initially evaluated by conventional PCR. The protocols for absolute quantitative real-time PCR (qPCR) were then adapted to validate the technique for the identification and quantification of GBS isolated by real-time detection of amplicons using fluorescence measurements. Finally, an infectivity test was conducted in tilapia infected with GBS strains. Total DNA from the host brain was subjected to the same technique, and the strains were re-isolated to validate Koch's postulates. The assay showed 100% specificity for the other bacterial species evaluated and a sensitivity of 367 gene copies per 20mg of brain tissue within 4h, making this test a valuable tool for health monitoring programs.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.format.extent1-25-
dc.language.isoeng-
dc.publisherElsevier B. V.-
dc.sourcePubMed-
dc.subjectBrazilen
dc.subjectCamp factoren
dc.subjectStreptococcosisen
dc.subjectTilapiaen
dc.titleValidation of absolute quantitative real-time PCR for the diagnosis of streptococcus agalactiae in fishen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationCentro de Aquicultura da UNESP - Univ Estadual Paulista, Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, SP CEP 14.884-900.-
dc.description.affiliationFaculdade de Ciência Agrárias e Veterinárias, Department of Technology, UNESP - Univ Estadual Paulista, Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, SP CEP 14.884-900.-
dc.description.affiliationCentro de Aquicultura da UNESP - Univ Estadual Paulista, Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, SP CEP 14.884-900. Electronic address: fabianap@caunesp.unesp.br.-
dc.description.affiliationUnespCentro de Aquicultura da UNESP - Univ Estadual Paulista, Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, SP CEP 14.884-900.-
dc.description.affiliationUnespFaculdade de Ciência Agrárias e Veterinárias, Department of Technology, UNESP - Univ Estadual Paulista, Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, SP CEP 14.884-900.-
dc.description.affiliationUnespCentro de Aquicultura da UNESP - Univ Estadual Paulista, Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, SP CEP 14.884-900.-
dc.description.sponsorshipIdFAPESP: 2011/07951-5-
dc.identifier.doi10.1016/j.mimet.2015.10.021-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal Of Microbiological Methods-
dc.identifier.pubmed26519771-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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