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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/13479
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dc.contributor.authorCarvalho, Armando de Mattos-
dc.contributor.authorAlves, Ana Liz Garcia-
dc.contributor.authorGolim, Marjorie Assis-
dc.contributor.authorMoroz, Andrei-
dc.contributor.authorHussni, Carlos Alberto-
dc.contributor.authorGomes de Oliveira, Patricia Galvao-
dc.contributor.authorDeffune, Elenice-
dc.date.accessioned2014-05-20T13:38:51Z-
dc.date.accessioned2016-10-25T16:54:54Z-
dc.date.available2014-05-20T13:38:51Z-
dc.date.available2016-10-25T16:54:54Z-
dc.date.issued2009-12-15-
dc.identifierhttp://dx.doi.org/10.1016/j.vetimm.2009.06.014-
dc.identifier.citationVeterinary Immunology and Immunopathology. Amsterdam: Elsevier B.V., v. 132, n. 2-4, p. 303-306, 2009.-
dc.identifier.issn0165-2427-
dc.identifier.urihttp://hdl.handle.net/11449/13479-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/13479-
dc.description.abstractThe purpose of this work was to isolate and cultivate mesenchymal stem cells (MSC) derived from equine adipose tissue and conduct cellular characterization with the following markers: CD90, CD44 and CD13. Adipose tissue collection was performed at the base of the horses' tails, followed by immediate isolation and cultivation of the MSC and posterior characterization by flow cytometry for the interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), fluorescein isothiocyanate (FITC), and tests with specific mAb mouse anti-horse CD13 and mouse anti-horse CD44. The technique used for isolation and cell cultivation proved to be safe and viable. The CD90 mAb expressed cross-reaction with MSC derived from equine adipose tissue and CD44 showed greater expression in cells as the number of culture passages increased. Although marker CD13 expresses reaction in other studies involving MSC in different species, it presented no expression in the experiment realized. The results obtained revealed the immunophenotypic characterization of the surface of isolated and cultivated MSC, classifying these cells as a promising type of progenitor cells that can be applied in equine cellular therapy. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.format.extent303-306-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectImmunophenotypic characterizationen
dc.subjectEquineen
dc.subjectAdipose tissueen
dc.subjectMesenchymal stem cellsen
dc.titleIsolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissueen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationSão Paulo State Univ, Dept Vet Surg & Anesthesiol, Sch Vet Med & Anim Sci, São Paulo, Brazil-
dc.description.affiliationSão Paulo State Univ, Botucatu Med Sch, Ctr Blood, São Paulo, Brazil-
dc.description.affiliationUnespSão Paulo State Univ, Dept Vet Surg & Anesthesiol, Sch Vet Med & Anim Sci, São Paulo, Brazil-
dc.description.affiliationUnespSão Paulo State Univ, Botucatu Med Sch, Ctr Blood, São Paulo, Brazil-
dc.description.sponsorshipIdFAPESP: 07-53496-2-
dc.identifier.doi10.1016/j.vetimm.2009.06.014-
dc.identifier.wosWOS:000272861100031-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofVeterinary Immunology and Immunopathology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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