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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/14606
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dc.contributor.authorLima-Neto, J. F.-
dc.contributor.authorFernandes, C. B.-
dc.contributor.authorAlvarenga, Marco Antonio-
dc.contributor.authorGolim, M. A.-
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz-
dc.date.accessioned2013-09-30T18:28:36Z-
dc.date.accessioned2014-05-20T13:42:03Z-
dc.date.accessioned2016-10-25T16:56:58Z-
dc.date.available2013-09-30T18:28:36Z-
dc.date.available2014-05-20T13:42:03Z-
dc.date.available2016-10-25T16:56:58Z-
dc.date.issued2010-08-01-
dc.identifierhttp://dx.doi.org/10.1007/s10561-009-9131-6-
dc.identifier.citationCell and Tissue Banking. Dordrecht: Springer, v. 11, n. 3, p. 261-268, 2010.-
dc.identifier.issn1389-9333-
dc.identifier.urihttp://hdl.handle.net/11449/14606-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/14606-
dc.description.abstractThis experiment aimed to study equine fibroblasts in culture analyzing and the cell cycle and viability of cells pre- and post-freezing. Skin fragments were obtained from 6 horses and cultured in DMEM high glucose + 10% FCS in 5% CO(2) until the beginning of confluence. Two passages were performed before freezing. Cells subjected to serum starvation (0.5% FCS) were analyzed for viability and cell cycle at 24, 48, 72, 96, 120, 144 and 168 h of culture. For the confluent groups, cells were analyzed at the moment they achieved confluence. Cellular viability was assisted with Hoescht 33342 and propidium iodide. The analysis of apoptosis/necrosis and cell cycle was performed using a flow cytometer (FACS Calibur BD(A (R))) after staining the cells with annexin V and propidium iodide. Both optical microscopy and flow cytometry confirmed that cellular viability was similar for serum starvation and confluent groups (average 84%). Similarly, both methods were efficient to synchronize the cell cycle before freezing. However, after thawing, serum starvation, for more than 24 h, was superior to culture for synchronizing cells in G0/G1 (69% x 90%). The results of this experiment indicate that equine fibroblasts can be efficiently cultured after thawing.en
dc.format.extent261-268-
dc.language.isoeng-
dc.publisherSpringer-
dc.sourceWeb of Science-
dc.subjectCell culture Fibroblastsen
dc.subjectFlow cytometeren
dc.subjectCell cycleen
dc.subjectAnnexin Ven
dc.subjectApoptosisen
dc.subjectEquineen
dc.titleViability and cell cycle analysis of equine fibroblasts cultured in vitroen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUNESP, FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUNESP, FM, Hemoctr, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP, FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP, FM, Hemoctr, BR-18618000 Botucatu, SP, Brazil-
dc.identifier.doi10.1007/s10561-009-9131-6-
dc.identifier.wosWOS:000279295000006-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofCell and Tissue Banking-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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