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http://acervodigital.unesp.br/handle/11449/14606
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DC Field | Value | Language |
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dc.contributor.author | Lima-Neto, J. F. | - |
dc.contributor.author | Fernandes, C. B. | - |
dc.contributor.author | Alvarenga, Marco Antonio | - |
dc.contributor.author | Golim, M. A. | - |
dc.contributor.author | Landim-Alvarenga, Fernanda da Cruz | - |
dc.date.accessioned | 2013-09-30T18:28:36Z | - |
dc.date.accessioned | 2014-05-20T13:42:03Z | - |
dc.date.accessioned | 2016-10-25T16:56:58Z | - |
dc.date.available | 2013-09-30T18:28:36Z | - |
dc.date.available | 2014-05-20T13:42:03Z | - |
dc.date.available | 2016-10-25T16:56:58Z | - |
dc.date.issued | 2010-08-01 | - |
dc.identifier | http://dx.doi.org/10.1007/s10561-009-9131-6 | - |
dc.identifier.citation | Cell and Tissue Banking. Dordrecht: Springer, v. 11, n. 3, p. 261-268, 2010. | - |
dc.identifier.issn | 1389-9333 | - |
dc.identifier.uri | http://hdl.handle.net/11449/14606 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/14606 | - |
dc.description.abstract | This experiment aimed to study equine fibroblasts in culture analyzing and the cell cycle and viability of cells pre- and post-freezing. Skin fragments were obtained from 6 horses and cultured in DMEM high glucose + 10% FCS in 5% CO(2) until the beginning of confluence. Two passages were performed before freezing. Cells subjected to serum starvation (0.5% FCS) were analyzed for viability and cell cycle at 24, 48, 72, 96, 120, 144 and 168 h of culture. For the confluent groups, cells were analyzed at the moment they achieved confluence. Cellular viability was assisted with Hoescht 33342 and propidium iodide. The analysis of apoptosis/necrosis and cell cycle was performed using a flow cytometer (FACS Calibur BD(A (R))) after staining the cells with annexin V and propidium iodide. Both optical microscopy and flow cytometry confirmed that cellular viability was similar for serum starvation and confluent groups (average 84%). Similarly, both methods were efficient to synchronize the cell cycle before freezing. However, after thawing, serum starvation, for more than 24 h, was superior to culture for synchronizing cells in G0/G1 (69% x 90%). The results of this experiment indicate that equine fibroblasts can be efficiently cultured after thawing. | en |
dc.format.extent | 261-268 | - |
dc.language.iso | eng | - |
dc.publisher | Springer | - |
dc.source | Web of Science | - |
dc.subject | Cell culture Fibroblasts | en |
dc.subject | Flow cytometer | en |
dc.subject | Cell cycle | en |
dc.subject | Annexin V | en |
dc.subject | Apoptosis | en |
dc.subject | Equine | en |
dc.title | Viability and cell cycle analysis of equine fibroblasts cultured in vitro | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | UNESP, FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618000 Botucatu, SP, Brazil | - |
dc.description.affiliation | UNESP, FM, Hemoctr, BR-18618000 Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618000 Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, FM, Hemoctr, BR-18618000 Botucatu, SP, Brazil | - |
dc.identifier.doi | 10.1007/s10561-009-9131-6 | - |
dc.identifier.wos | WOS:000279295000006 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Cell and Tissue Banking | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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