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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/16274
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dc.contributor.authorHerrera, Bruno S.-
dc.contributor.authorMartins-Porto, Rodrigo-
dc.contributor.authorMaia-Dantas, Aline-
dc.contributor.authorCampi, Paula-
dc.contributor.authorSpolidório, Luis Carlos-
dc.contributor.authorCosta, Soraia K. P.-
dc.contributor.authorVan Dyke, Thomas E.-
dc.contributor.authorGyurko, Robert-
dc.contributor.authorMuscara, Marcelo N.-
dc.date.accessioned2014-05-20T13:46:05Z-
dc.date.accessioned2016-10-25T16:59:55Z-
dc.date.available2014-05-20T13:46:05Z-
dc.date.available2016-10-25T16:59:55Z-
dc.date.issued2011-11-01-
dc.identifierhttp://dx.doi.org/10.1902/jop.2011.100768-
dc.identifier.citationJournal of Periodontology. Chicago: Amer Acad Periodontology, v. 82, n. 11, p. 1608-1615, 2011.-
dc.identifier.issn0022-3492-
dc.identifier.urihttp://hdl.handle.net/11449/16274-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/16274-
dc.description.abstractBackground: Inflammatory stimuli activate inducible nitric oxide synthase (iNOS) in a variety of cell types, including osteoclasts (OC) and osteoblasts, resulting in sustained NO production. In this study, we evaluate the alveolar bone loss in rats with periodontitis under long-term iNOS inhibition, and the differentiation and activity of OC from iNOS-knockout (KO) mice in vitro.Methods: Oral aminoguanidine (an iNOS inhibitor) or water treatment was started 2 weeks before induction of periodontitis. Rats were sacrificed 3, 7, or 14 days after ligature placement, and alveolar bone loss was evaluated. In vitro OC culture experiments were also performed to study the differentiation of freshly isolated bone marrow cells from both iNOS KO and wild-type C57BL/6 mice. OC were counted 6 days later after tartrate-resistant acid phosphatase staining (a marker of osteoclast identity), and bone resorption activity was assessed by counting the number of resorption pits on dentin disks.Results: Rats with ligature showed progressive and significant alveolar bone loss compared to sham animals, and aminoguanidine treatment significantly inhibited ligature-induced bone loss at 7 and 14 days after the induction. In comparison to bone marrow cells from wild-type mice, cells from iNOS KO mice showed decreased OC growth and the resulting OC covered a smaller culture dish area and generated fewer resorption pit counts.Conclusion: Our results demonstrate that iNOS inhibition prevents alveolar bone loss in a rat model of ligature-induced periodontitis, thus confirming that iNOS-derived NO plays a crucial role in the pathogenesis of periodontitis, probably by stimulating OC differentiation and activity. J Periodontol 2011;82:1608-1615.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.description.sponsorshipNational Institute of Dental and Craniofacial Research from National Institutes of Health, Bethesda, Maryland-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent1608-1615-
dc.language.isoeng-
dc.publisherAmer Acad Periodontology-
dc.sourceWeb of Science-
dc.subjectAnimal experimental useen
dc.subjectbone resorptionen
dc.subjectiNOS enzymeen
dc.subjectnitric oxideen
dc.subjectosteoclastsen
dc.subjectperiodontitisen
dc.titleiNOS-Derived Nitric Oxide Stimulates Osteoclast Activity and Alveolar Bone Loss in Ligature-Induced Periodontitis in Ratsen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionBoston University-
dc.description.affiliationState Univ São Paulo, Dept Physiol & Pathol, Araraquara Dent Sch, BR-14801590 Araraquara, SP, Brazil-
dc.description.affiliationBoston Univ, Dept Periodontol & Oral Biol, Goldman Sch Dent Med, Boston, MA 02215 USA-
dc.description.affiliationUnespState Univ São Paulo, Dept Physiol & Pathol, Araraquara Dent Sch, BR-14801590 Araraquara, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 02/10087-1-
dc.description.sponsorshipIdFAPESP: 08/02893-4-
dc.description.sponsorshipIdCoordination for the Improvement of Higher Level -or Education-Personnel, Brasilia, Brazil: 1154-05-2-
dc.description.sponsorshipIdNIDCR from NIH, Bethesda, Maryland: DE-16922-
dc.description.sponsorshipIdNIDCR from NIH, Bethesda, Maryland: DE-15566-
dc.identifier.doi10.1902/jop.2011.100768-
dc.identifier.wosWOS:000297029000015-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofJournal of Periodontology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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