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DC Field | Value | Language |
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dc.contributor.author | Sugizaki, Mario Mateus | - |
dc.contributor.author | Leopoldo, Andre Soares | - |
dc.contributor.author | Okoshi, Marina Politi | - |
dc.contributor.author | Bruno, Alessandro | - |
dc.contributor.author | Conde, Sandro Jose | - |
dc.contributor.author | Lima-Leopoldo, Ana Paula | - |
dc.contributor.author | Padovani, Carlos Roberto | - |
dc.contributor.author | Carvalho, Robson Francisco | - |
dc.contributor.author | do Nascimento, Andre Ferreira | - |
dc.contributor.author | Salome de Campos, Dijon Henrique | - |
dc.contributor.author | Nogueira, Célia Regina | - |
dc.contributor.author | Cicogna, Antonio Carlos | - |
dc.date.accessioned | 2014-05-20T13:48:09Z | - |
dc.date.accessioned | 2016-10-25T17:01:11Z | - |
dc.date.available | 2014-05-20T13:48:09Z | - |
dc.date.available | 2016-10-25T17:01:11Z | - |
dc.date.issued | 2009-09-01 | - |
dc.identifier | http://dx.doi.org/10.1139/Y09-060 | - |
dc.identifier.citation | Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 87, n. 9, p. 666-673, 2009. | - |
dc.identifier.issn | 0008-4212 | - |
dc.identifier.uri | http://hdl.handle.net/11449/17174 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/17174 | - |
dc.description.abstract | Previous studies have shown that food restriction promotes myocardial dysfunction in rats. However, the molecular mechanisms that are responsible are unclear. We investigated the role of sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2) on myocardial performance in food-restricted rats. Male Wistar-Kyoto rats, 60 days old, were fed a control or restricted diet (daily energy intake reduced to 50% of the control) for 90 days. Expression of Serca2a, phospholamban (PLB), Na(+)/Ca(2+) exchanger (NCX), and thyroid hormone receptor (TR alpha 1, TR beta 1) mRNA was determined by quantitative PCR. SERCA2 activity was measured by using 20 mu mol/L cyclopiazonic acid (CPA) in a left ventricular papillary muscle preparation during isometric contraction in basal conditions and during post-rest contraction. Serum concentrations of thyroxine (T(4)) and thyrotropin (TSH) were also determined. The 50%-restricted diet reduced body and ventricular weight and serum T4 and TSH levels. The interaction of CPA and food restriction reduced peak developed tension and maximum rate of tension decline (-dT/dt), but increased the resting tension intensity response during post-rest contraction. PLB and NCX mRNA were upregulated and TR alpha 1 mRNA was downregulated by food restriction. These results suggest that food restriction promotes myocardial dysfunction related to impairment of sarcoplasmic reticulum Ca(2+) uptake as a result of a hypothyroid state. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Faculty of Medicine, UNESP-Botucatu | - |
dc.format.extent | 666-673 | - |
dc.language.iso | eng | - |
dc.publisher | Natl Research Council Canada-n R C Research Press | - |
dc.source | Web of Science | - |
dc.subject | SERCA2 | en |
dc.subject | cyclopiazonic acid | en |
dc.subject | calcium handling | en |
dc.subject | food restriction | en |
dc.subject | rats | en |
dc.subject | myocardial function | en |
dc.subject | thyroid hormone | en |
dc.title | Severe food restriction induces myocardial dysfunction related to SERCA2 activity | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Fac Integradas Bauru | - |
dc.description.affiliation | UNESP State Univ Julio Mesquita Filho, Sch Med, Dept Clin & Cardiol, São Paulo, Brazil | - |
dc.description.affiliation | UNESP State Univ Julio Mesquita Filho, Dept Biostat, Inst Biol Sci, São Paulo, Brazil | - |
dc.description.affiliation | UNESP State Univ Julio Mesquita Filho, Dept Morphol, Inst Biol Sci, São Paulo, Brazil | - |
dc.description.affiliation | Fac Integradas Bauru, Dept Phys Educ, FIB, São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP State Univ Julio Mesquita Filho, Sch Med, Dept Clin & Cardiol, São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP State Univ Julio Mesquita Filho, Dept Biostat, Inst Biol Sci, São Paulo, Brazil | - |
dc.description.affiliationUnesp | UNESP State Univ Julio Mesquita Filho, Dept Morphol, Inst Biol Sci, São Paulo, Brazil | - |
dc.description.sponsorshipId | FAPESP: 02/12314-5 | - |
dc.description.sponsorshipId | CNPq: 302502/2004-1 | - |
dc.identifier.doi | 10.1139/Y09-060 | - |
dc.identifier.wos | WOS:000271315700002 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Canadian Journal of Physiology and Pharmacology | - |
dc.identifier.orcid | 0000-0002-4901-7714 | pt |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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