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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/17212
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dc.contributor.authorFerrarese, MLL-
dc.contributor.authorRodrigues, J. D.-
dc.contributor.authorFerrarese, O.-
dc.date.accessioned2014-05-20T13:48:17Z-
dc.date.accessioned2016-10-25T17:01:13Z-
dc.date.available2014-05-20T13:48:17Z-
dc.date.available2016-10-25T17:01:13Z-
dc.date.issued2000-03-01-
dc.identifierhttp://dx.doi.org/10.1055/s-2000-9162-
dc.identifier.citationPlant Biology. Stuttgart: Georg Thieme Verlag, v. 2, n. 2, p. 152-153, 2000.-
dc.identifier.issn1435-8603-
dc.identifier.urihttp://hdl.handle.net/11449/17212-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/17212-
dc.description.abstractThe high performance liquid chromatography (HPLC) technique was applied to measure phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity in soybean (Glycine max L. Merril cv. BR16) roots. t-Cinnamate, the catalytic product of the PAL reaction was quantified at 275 nm by isocratic elution with methanol:water through an ODS(M) column. Comparative experiments were carried out with 1.0 mM ferulic acid, an inducer of PAL activity. The results suggest that liquid chromatography is a rapid and sensitive method to analyze PAL activity in non-purified extract.en
dc.format.extent152-153-
dc.language.isoeng-
dc.publisherGeorg Thieme Verlag-
dc.sourceWeb of Science-
dc.subjectt-cinnamatept
dc.subjectHPLCpt
dc.subjectPALpt
dc.subjectsoybeanpt
dc.titlePhenylalanine ammonia-lyase activity in soybean roots extract measured by reverse-phase high performance liquid chromatographyen
dc.typeoutro-
dc.contributor.institutionUniv Maringa-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Maringa, Dept Biochem, BR-87020900 Maringa, Parana, Brazil-
dc.description.affiliationUNESP, IB, Dept Bot, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP, IB, Dept Bot, BR-18618000 Botucatu, SP, Brazil-
dc.identifier.doi10.1055/s-2000-9162-
dc.identifier.wosWOS:000086431400004-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofPlant Biology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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