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dc.contributor.authorSartorello, Robson-
dc.contributor.authorBudu, Alexandre-
dc.contributor.authorBagnaresi, Piero-
dc.contributor.authorFernandes, Carlos A. H.-
dc.contributor.authorSato, Paloma M.-
dc.contributor.authorBueno, Vania B.-
dc.contributor.authorFontes, Marcos R. M.-
dc.contributor.authorOliveira, Pedro L.-
dc.contributor.authorPaiva-Silva, Gabriela O.-
dc.contributor.authorAlves, Simone V.-
dc.contributor.authorNetto, Luis E. S.-
dc.contributor.authorCatalani, Luiz H.-
dc.contributor.authorGarcia, Celia R. S.-
dc.identifier.citationCell Biology International. London: Portland Press Ltd, v. 34, n. 8, p. 859-865, 2010.-
dc.description.abstractThe cellular traffic of haem during the development of the human malaria parasite Plasmodium falciparum, through the stages R (ring), T (trophozoite) and S (schizonts), was investigated within RBC (red blood cells). When Plasmodium cultures were incubated with a fluorescent haem analogue, ZnPPIX (Zn protoporphyrin IX) the probe was seen at the cytoplasm (R stage), and the vesicle-like structure distribution pattern was more evident at T and S stages. The temporal sequence of ZnPPIX uptake by P. falciparum-infected erythrocytes shows that at R and S stages, a time-increase acquisition of the porphyrin reaches the maximum fluorescence distribution after 60 min; in contrast, at the T stage, the maximum occurs after 120 min of ZnPPIX uptake. The difference in time-increase acquisition of the porphyrin is in agreement with a maximum activity of haem uptake at the T stage. To gain insights into haem metabolism, recombinant PfHO (P. falciparum haem oxygenase) was expressed, and the conversion of haem into BV (biliverdin) was detected. These findings point out that, in addition to haemozoin formation, the malaria parasite P. falciparum has evolved two distinct mechanisms for dealing with haem toxicity, namely, the uptake of haem into a cellular compartment where haemozoin is formed and HO activity. However, the low Plasmodium HO activity detected reveals that the enzyme appears to be a very inefficient way to scavenge the haem compared with the Plasmodium ability to uptake the haem analogue ZnPPIX and delivering it to the food vacuole.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.publisherPortland Press-
dc.sourceWeb of Science-
dc.subjectcellular trafficen
dc.subjectconfocal microscopyen
dc.subjecthaem oxygenaseen
dc.titleIn vivo uptake of a haem analogue Zn protoporphyrin IX by the human malaria parasite P. falciparum-infected red blood cellsen
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)-
dc.description.affiliationUniv São Paulo, Dept Fisiol, Inst Biociencias, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUniv São Paulo, Dept Parasitol, Inst Ciencias Biomed, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUniv São Paulo, Dept Quim Fundamental, Inst Quim, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUniv Estadual Paulista Julio de Mesquite Filho, Dept Fis & Biofis, Inst Biociencias, Botucatu, SP, Brazil-
dc.description.affiliationUniv Fed Rio de Janeiro, Inst Bioquim Med, Rio de Janeiro, Brazil-
dc.description.affiliationUniv São Paulo, Dept Biol, Inst Biociencias, BR-05508900 São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista Julio de Mesquite Filho, Dept Fis & Biofis, Inst Biociencias, Botucatu, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofCell Biology International-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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