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dc.contributor.authorSiqueira Neto, J. L.-
dc.contributor.authorLira, C. B. B.-
dc.contributor.authorGiardini, M. A.-
dc.contributor.authorKhater, L.-
dc.contributor.authorPerez, A. M.-
dc.contributor.authorPeroni, L. A.-
dc.contributor.authordos Reis, J. R. R.-
dc.contributor.authorFreitas-Junior, L. H.-
dc.contributor.authorRamos, C. H. I.-
dc.contributor.authorCano, M. I. N.-
dc.date.accessioned2014-05-20T13:50:12Z-
dc.date.accessioned2016-10-25T17:02:25Z-
dc.date.available2014-05-20T13:50:12Z-
dc.date.available2016-10-25T17:02:25Z-
dc.date.issued2007-06-29-
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2007.04.144-
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 358, n. 2, p. 417-423, 2007.-
dc.identifier.issn0006-291X-
dc.identifier.urihttp://hdl.handle.net/11449/17932-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/17932-
dc.description.abstractReplication protein A (RPA) is a highly conserved heterotrimeric single-stranded DNA-binding protein involved in different events of DNA metabolism. In yeast, subunits 1 (RPA-1) and 2 (RPA-2) work also as telomerase recruiters and, in humans, the complex unfolds G-quartet structures formed by the 3' G-rich telomeric strand. In most eukaryotes, RPA-1 and RPA-2 bind DNA using multiple OB fold domains. In trypanosomatids, including Leishmania, RPA-1 has a canonical OB fold and a truncated RFA-1 structural domain. In Leishmania amazonensis, RPA-1 alone can form a complex in vitro with the telomeric G-rich strand. In this work, we show that LaRPA-1 is a nuclear protein that associates in vivo with Leishmania telomeres. We mapped the boundaries of the OB fold DNA-binding domain using deletion mutants. Since Leishmania and other trypanosomatids lack homologues of known telomere end binding proteins, our results raise questions about the function of RPA-1 in parasite telomeres. (C) 2007 Elsevier B.V. All rights reserved.en
dc.format.extent417-423-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectreplication protein A-1pt
dc.subjectLeishmania amazonensispt
dc.subjectOB foldpt
dc.subjecttelomere end binding proteinpt
dc.subjectimmunolocalizationpt
dc.titleLeishmania replication protein A-1 binds in vivo single-stranded telomeric DNAen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionInst Pasteur-
dc.description.affiliationUNESP, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Campinas, SP, Brazil-
dc.description.affiliationUniv Estadual Campinas, Inst Quim, Campinas, SP, Brazil-
dc.description.affiliationInst Pasteur, Seoul, South Korea-
dc.description.affiliationUnespUNESP, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil-
dc.identifier.doi10.1016/j.bbrc.2007.04.144-
dc.identifier.wosWOS:000246927300007-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofBiochemical and Biophysical Research Communications-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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