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http://acervodigital.unesp.br/handle/11449/17932
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DC Field | Value | Language |
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dc.contributor.author | Siqueira Neto, J. L. | - |
dc.contributor.author | Lira, C. B. B. | - |
dc.contributor.author | Giardini, M. A. | - |
dc.contributor.author | Khater, L. | - |
dc.contributor.author | Perez, A. M. | - |
dc.contributor.author | Peroni, L. A. | - |
dc.contributor.author | dos Reis, J. R. R. | - |
dc.contributor.author | Freitas-Junior, L. H. | - |
dc.contributor.author | Ramos, C. H. I. | - |
dc.contributor.author | Cano, M. I. N. | - |
dc.date.accessioned | 2014-05-20T13:50:12Z | - |
dc.date.accessioned | 2016-10-25T17:02:25Z | - |
dc.date.available | 2014-05-20T13:50:12Z | - |
dc.date.available | 2016-10-25T17:02:25Z | - |
dc.date.issued | 2007-06-29 | - |
dc.identifier | http://dx.doi.org/10.1016/j.bbrc.2007.04.144 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 358, n. 2, p. 417-423, 2007. | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/11449/17932 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/17932 | - |
dc.description.abstract | Replication protein A (RPA) is a highly conserved heterotrimeric single-stranded DNA-binding protein involved in different events of DNA metabolism. In yeast, subunits 1 (RPA-1) and 2 (RPA-2) work also as telomerase recruiters and, in humans, the complex unfolds G-quartet structures formed by the 3' G-rich telomeric strand. In most eukaryotes, RPA-1 and RPA-2 bind DNA using multiple OB fold domains. In trypanosomatids, including Leishmania, RPA-1 has a canonical OB fold and a truncated RFA-1 structural domain. In Leishmania amazonensis, RPA-1 alone can form a complex in vitro with the telomeric G-rich strand. In this work, we show that LaRPA-1 is a nuclear protein that associates in vivo with Leishmania telomeres. We mapped the boundaries of the OB fold DNA-binding domain using deletion mutants. Since Leishmania and other trypanosomatids lack homologues of known telomere end binding proteins, our results raise questions about the function of RPA-1 in parasite telomeres. (C) 2007 Elsevier B.V. All rights reserved. | en |
dc.format.extent | 417-423 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | replication protein A-1 | pt |
dc.subject | Leishmania amazonensis | pt |
dc.subject | OB fold | pt |
dc.subject | telomere end binding protein | pt |
dc.subject | immunolocalization | pt |
dc.title | Leishmania replication protein A-1 binds in vivo single-stranded telomeric DNA | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | - |
dc.contributor.institution | Inst Pasteur | - |
dc.description.affiliation | UNESP, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Campinas, Inst Biol, Campinas, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Campinas, Inst Quim, Campinas, SP, Brazil | - |
dc.description.affiliation | Inst Pasteur, Seoul, South Korea | - |
dc.description.affiliationUnesp | UNESP, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.04.144 | - |
dc.identifier.wos | WOS:000246927300007 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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