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DC Field | Value | Language |
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dc.contributor.author | da Silva, Eder Marques | - |
dc.contributor.author | Wong, Marina Sek Lien | - |
dc.contributor.author | Martins, Cesar | - |
dc.contributor.author | Wasko, Adriane Pinto | - |
dc.date.accessioned | 2014-05-20T13:50:20Z | - |
dc.date.accessioned | 2016-10-25T17:02:29Z | - |
dc.date.available | 2014-05-20T13:50:20Z | - |
dc.date.available | 2016-10-25T17:02:29Z | - |
dc.date.issued | 2012-10-01 | - |
dc.identifier | http://dx.doi.org/10.1007/s10695-012-9638-9 | - |
dc.identifier.citation | Fish Physiology and Biochemistry. Dordrecht: Springer, v. 38, n. 5, p. 1487-1496, 2012. | - |
dc.identifier.issn | 0920-1742 | - |
dc.identifier.uri | http://hdl.handle.net/11449/17963 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/17963 | - |
dc.description.abstract | The matrinch Brycon amazonicus, a commercially important freshwater fish resource, has no heteromorphic sex chromosomes so far described. In the present study, we performed a screening of sex-associated DNA markers in this species, through the use of a random amplified polymorphic DNA (RAPD) assay and a genomic DNA restriction digestion analysis. DNA digestions evidenced no differences between sexes. Sixty-six random primers were used in pooled and individual DNA samples of males and females, and the analysis of the RAPD fingerprints revealed one female sex-associated band. Cloning and sequencing of this band led to the identification of two distinct DNA segments. While one of the isolated fragments showed a significant identity with a described protein gene (phosphatidylinositol glycan anchor biosynthesis, class W), the other fragment, composed of 535 bp, corresponds to a novel DNA marker. Further experiments were performed with this second DNA fragment in order to verify its sex-specificity. Data on dot blot hybridization, using total DNA of both sexes, confirmed its female-specificity in B. amazonicus. A primer set was designed based on its sequence data and used in PCR with DNA samples of this species, leading to diagnose the animals' sexes with a 100 % overall accuracy through a sequence characterized amplified region approach. No amplification results were found for two other species of the genus-B. orbignyanus and B. lundii. The obtained data can lead to the hypothesis that B. amazonicus may present heteromorphic sex chromosomes that should be in an early phase of differentiation. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
dc.format.extent | 1487-1496 | - |
dc.language.iso | eng | - |
dc.publisher | Springer | - |
dc.source | Web of Science | - |
dc.subject | Brycon | en |
dc.subject | RAPD-PCR | en |
dc.subject | Enzyme digestion | en |
dc.subject | Sex-specific DNA marker | en |
dc.subject | Sexing | en |
dc.title | Screening and characterization of sex-specific DNA fragments in the freshwater fish matrinch, Brycon amazonicus (Teleostei: Characiformes: Characidae) | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ Estadual Paulista UNESP, Dept Genet, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista UNESP, Dept Morfol, Inst Biociencias, Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista UNESP, Dept Genet, Inst Biociencias, BR-18618970 Botucatu, SP, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista UNESP, Dept Morfol, Inst Biociencias, Botucatu, SP, Brazil | - |
dc.identifier.doi | 10.1007/s10695-012-9638-9 | - |
dc.identifier.wos | WOS:000308968000023 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Fish Physiology and Biochemistry | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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