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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/18570
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dc.contributor.authorPinhal, Danillo-
dc.contributor.authorGadig, Otto Bismarck Fazzano-
dc.contributor.authorMartins, Cesar-
dc.date.accessioned2014-05-20T13:51:59Z-
dc.date.accessioned2016-10-25T17:03:13Z-
dc.date.available2014-05-20T13:51:59Z-
dc.date.available2016-10-25T17:03:13Z-
dc.date.issued2009-12-01-
dc.identifierhttp://dx.doi.org/10.1007/s12686-009-9008-9-
dc.identifier.citationConservation Genetics Resources. Dordrecht: Springer, v. 1, n. 1, p. 35-38, 2009.-
dc.identifier.issn1877-7252-
dc.identifier.urihttp://hdl.handle.net/11449/18570-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/18570-
dc.description.abstractA molecular approach based on nuclear 5S rDNA sequence variability was applied successfully to correctly identify samples from the two Rhizoprionodon species collected in the wild or sold in markets. The sequence of the non-transcribed spacer (NTS) of the 5S rDNA showed high interspecific variability and no intra-specific polymorphism, making it a useful marker for sharpnose shark identification. Polymorphisms in the NTS sequences of Rhizoprionodon sharks also created unique restriction patterns for each species after PCR-RFLP analysis. The 5S rDNA polymorphism represents a fast and non expensive tool to access species identification when rapid and unequivocal identification of shark products is needed, particularly for future management and other investigations.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.format.extent35-38-
dc.language.isoeng-
dc.publisherSpringer-
dc.sourceWeb of Science-
dc.subjectGenetic markersen
dc.subjectNon-transcribed spaceren
dc.subjectTrade monitoringen
dc.subjectSharksen
dc.subjectSpecies identificationen
dc.titleGenetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNAen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Morfol, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUNESP Univ Estadual Paulista, Sao Vicente, SP, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Morfol, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sao Vicente, SP, Brazil-
dc.identifier.doi10.1007/s12686-009-9008-9-
dc.identifier.wosWOS:000207922400009-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofConservation Genetics Resources-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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