You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRocha, Thalita-
dc.contributor.authorSoares de Barros, Luciano Libardi-
dc.contributor.authorFontana, Karina-
dc.contributor.authorde Souza, Bibiana Monson-
dc.contributor.authorPalma, Mario Sergio-
dc.contributor.authorda Cruz-Hoefling, Maria Alice-
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 55, n. 7, p. 1213-1221, 2010.-
dc.description.abstractMastoparan firstly described as an inducer of mast cell granules exocytosis has been also related to many essential mechanisms of cell function. In skeletal muscle tissue the best characterization of mastoparan effect was induction of myonecrosis. We examined the ability of mastoparan Polybia-MPII from Polybia paulista wasp venom to induce apoptosis and inflammation in mouse tibial anterior muscle. The activation of caspase 3 and 9, the expression of INF-alpha, IFN-gamma, CD68 and CD163 proteins, specific of resident and migrant macrophages, respectively, were examined (3 h to 21 d). TUNEL-positive nuclei were found both in damaged and normal-looking muscle fibres, whereas the caspases, cytokines and macrophages proteins were only in damaged fibres. The caspase 3 and 9 expression and the immunolabelled areas of TNF-alpha and IFN-gamma were significantly higher compared to control. TUNEL-positive nuclei and TNF-alpha expression were also present in regenerating fibres. CD68 and CD163 signalize necrotic debris removal, release of chemo-attractants and cytokines which have been considered a pre-requisite for muscle regeneration. High levels of cytokines coincided with the intense muscle proteolysis by mastoparan (3-24 h) and the climax of regeneration (3 d) whereas cytokines decline corresponded to periods of tissue remodeling and intense fibre protein synthesis (7-21 d). We conclude that the mastoparan Polybia-mPII causes myonecrosis and apoptosis, the latter probably involving caspases signalling, corroborated by mitochondrial damage, and cytokines activation. (C) 2009 Elsevier Ltd. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
dc.publisherPergamon-Elsevier B.V. Ltd-
dc.sourceWeb of Science-
dc.subjectSkeletal muscleen
dc.subjectTUNEL reactionen
dc.titleInflammation and apoptosis induced by mastoparan Polybia-MPII on skeletal muscleen
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationState Univ Campinas UNICAMP, Inst Biol, Dept Histol & Embryol, BR-13083970 Campinas, SP, Brazil-
dc.description.affiliationIBRC UNESP, CEIS, Dept Biol, BR-13506900 Rio Claro, SP, Brazil-
dc.description.affiliationUnespIBRC UNESP, CEIS, Dept Biol, BR-13506900 Rio Claro, SP, Brazil-
dc.description.sponsorshipIdCNPq: 141336/2005-6-
dc.description.sponsorshipIdFAPESP: 05/53625-1-
dc.rights.accessRightsAcesso restrito-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.