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Proteomic analysis of kidney in rats chronically exposed to fluoride
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
  • Universidade Federal do Rio de Janeiro (UFRJ)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Sponsorship Process Number: 
  • FAPESP: 05/02744-0
  • FAPESP: 05/03273-1
  • CNPq: 307006/2006-9
Two-dimensional gel electrophoresis (2-DE) was used to better understand alterations in renal metabolism induced by fluoride (F). Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F for 60 days (n=6/group). Kidneys were collected for proteomic and histological (HE) analysis. After protein isolation, renal proteome profiles were examined using 2-DE and Colloidal Coomassie Blue staining. Protein spots with a 2-fold significant difference as detected by quantitative intensity analysis (image Master Platinum software) and t-test (p < 0.05) were excised and analyzed by MALDI-TOF MS (matrix assisted laser desorption ionization-time-of-flight mass spectrometry). The histological analysis revealed no damage in kidneys induced by F, except for a vascular congestion in the 50 ppm F group. Between control vs 50 ppm F, and control vs 5 ppm F groups, 12 and 6 differentially expressed proteins were detected, respectively. Six proteins, mainly related with metabolism, detoxification and housekeeping, were successfully identified. At the high F group, pyruvate carboxylase, a protein involved in the formation of oxaloacetate was found to be downregulated, while enoyl coenzyme A hydratase, involved in fatty acids oxidation, was found to be upregulated. Thus, proteomic analysis can provide new insights into the alterations in renal metabolism after F exposure, even in low doses. (C) 2009 Elsevier B.V. All rights reserved.
Issue Date: 
Chemico-biological Interactions. Clare: Elsevier B.V., v. 180, n. 2, p. 305-311, 2009.
Time Duration: 
Elsevier B.V.
  • Proteomics
  • Mass spectrometry
  • Two-dimensional gel electrophoresis
  • Fluoride
  • Kidney
  • Metabolism
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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