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http://acervodigital.unesp.br/handle/11449/20035
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DC Field | Value | Language |
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dc.contributor.author | Kobayashi, Claudia A. N. | - |
dc.contributor.author | Leite, Aline L. | - |
dc.contributor.author | Silva, Thelma L. | - |
dc.contributor.author | Santos, Lucilene D. | - |
dc.contributor.author | Nogueira, Fabio C. S. | - |
dc.contributor.author | Oliveiraa, Rodrigo C. | - |
dc.contributor.author | Palma, Mario Sergio | - |
dc.contributor.author | Domont, Gilberto B. | - |
dc.contributor.author | Buzalaf, Marilia A. R. | - |
dc.date.accessioned | 2014-05-20T13:56:01Z | - |
dc.date.accessioned | 2016-10-25T17:05:30Z | - |
dc.date.available | 2014-05-20T13:56:01Z | - |
dc.date.available | 2016-10-25T17:05:30Z | - |
dc.date.issued | 2009-07-15 | - |
dc.identifier | http://dx.doi.org/10.1016/j.cbi.2009.03.009 | - |
dc.identifier.citation | Chemico-biological Interactions. Clare: Elsevier B.V., v. 180, n. 2, p. 305-311, 2009. | - |
dc.identifier.issn | 0009-2797 | - |
dc.identifier.uri | http://hdl.handle.net/11449/20035 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/20035 | - |
dc.description.abstract | Two-dimensional gel electrophoresis (2-DE) was used to better understand alterations in renal metabolism induced by fluoride (F). Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F for 60 days (n=6/group). Kidneys were collected for proteomic and histological (HE) analysis. After protein isolation, renal proteome profiles were examined using 2-DE and Colloidal Coomassie Blue staining. Protein spots with a 2-fold significant difference as detected by quantitative intensity analysis (image Master Platinum software) and t-test (p < 0.05) were excised and analyzed by MALDI-TOF MS (matrix assisted laser desorption ionization-time-of-flight mass spectrometry). The histological analysis revealed no damage in kidneys induced by F, except for a vascular congestion in the 50 ppm F group. Between control vs 50 ppm F, and control vs 5 ppm F groups, 12 and 6 differentially expressed proteins were detected, respectively. Six proteins, mainly related with metabolism, detoxification and housekeeping, were successfully identified. At the high F group, pyruvate carboxylase, a protein involved in the formation of oxaloacetate was found to be downregulated, while enoyl coenzyme A hydratase, involved in fatty acids oxidation, was found to be upregulated. Thus, proteomic analysis can provide new insights into the alterations in renal metabolism after F exposure, even in low doses. (C) 2009 Elsevier B.V. All rights reserved. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.format.extent | 305-311 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Proteomics | en |
dc.subject | Mass spectrometry | en |
dc.subject | Two-dimensional gel electrophoresis | en |
dc.subject | Fluoride | en |
dc.subject | Kidney | en |
dc.subject | Metabolism | en |
dc.title | Proteomic analysis of kidney in rats chronically exposed to fluoride | en |
dc.type | outro | - |
dc.contributor.institution | Universidade de São Paulo (USP) | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Universidade Federal do Rio de Janeiro (UFRJ) | - |
dc.description.affiliation | Univ São Paulo, Bauru Dent Sch, Bauru, SP, Brazil | - |
dc.description.affiliation | Univ Estadual Paulista, Rio Claro Biosci Inst, Rio Claro, SP, Brazil | - |
dc.description.affiliation | Univ Fed Rio de Janeiro, Inst Chem, Rio de Janeiro, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Rio Claro Biosci Inst, Rio Claro, SP, Brazil | - |
dc.description.sponsorshipId | FAPESP: 05/02744-0 | - |
dc.description.sponsorshipId | FAPESP: 05/03273-1 | - |
dc.description.sponsorshipId | CNPq: 307006/2006-9 | - |
dc.identifier.doi | 10.1016/j.cbi.2009.03.009 | - |
dc.identifier.wos | WOS:000267584000021 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Chemico-biological Interactions | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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