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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/20060
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dc.contributor.authorGomes, E.-
dc.contributor.authorSerzedello, A.-
dc.contributor.authorDa Silva, R.-
dc.date.accessioned2014-05-20T13:56:05Z-
dc.date.accessioned2016-10-25T17:05:33Z-
dc.date.available2014-05-20T13:56:05Z-
dc.date.available2016-10-25T17:05:33Z-
dc.date.issued1998-01-01-
dc.identifierhttp://cat.inist.fr/?aModele=afficheN&cpsidt=1709661-
dc.identifier.citationMicrobios. Cambridge: Faculty Press, v. 96, n. 383, p. 39-50, 1998.-
dc.identifier.issn0026-2633-
dc.identifier.urihttp://hdl.handle.net/11449/20060-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/20060-
dc.description.abstractA RNase of Aspergillus flavipes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55 degrees C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3'-GMP from yeast RNA.en
dc.format.extent39-50-
dc.language.isoeng-
dc.publisherFaculty Press-
dc.sourceWeb of Science-
dc.subjectribonucleasept
dc.subjectAspergilluspt
dc.subjectnucleotidespt
dc.titlePurification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ : 1501)en
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Lab Bioquim Proc & Microbiol Aplicada, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, Dept Quim & Geosci, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUNESP, Dept Bioquim & Microbiol, Rio Claro, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Lab Bioquim Proc & Microbiol Aplicada, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Quim & Geosci, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUnespUNESP, Dept Bioquim & Microbiol, Rio Claro, SP, Brazil-
dc.identifier.wosWOS:000078475500004-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofMicrobios-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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