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http://acervodigital.unesp.br/handle/11449/20079
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DC Field | Value | Language |
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dc.contributor.author | Carmona, E. C. | - |
dc.contributor.author | Fialho, M. B. | - |
dc.contributor.author | Buchgnani, E. B. | - |
dc.contributor.author | Coelho, G. D. | - |
dc.contributor.author | Brocheto-Braga, M. R. | - |
dc.contributor.author | Jorge, J. A. | - |
dc.date.accessioned | 2014-05-20T13:56:07Z | - |
dc.date.accessioned | 2016-10-25T17:05:34Z | - |
dc.date.available | 2014-05-20T13:56:07Z | - |
dc.date.available | 2016-10-25T17:05:34Z | - |
dc.date.issued | 2005-01-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.procbio.2004.01.010 | - |
dc.identifier.citation | Process Biochemistry. Oxford: Elsevier B.V., v. 40, n. 1, p. 359-364, 2005. | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/11449/20079 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/20079 | - |
dc.description.abstract | A strain of Aspergillus versicolor produces a xylanolytic complex containing two components, the minor component being designated xylanase II. The highest production of xylanase II was observed in cultures grown for 5 days in 1% wheat bran as carbon source, at pH 6.5. Xylanase II was purified 28-fold by DEAE-Sephadex and HPLC GF-5 10 gel filtration. Xylanase II was a monomeric glycoprotein, exhibiting a molecular mass of 32 kDa with 14.1% of carbohydrate content. Optimal pH and temperature values for the enzyme activity were about 6.0-7.0 and 55 degreesC, respectively. Xylanase II thermoinactivation at 50degreesC showed a biphasic curve. The ions Hg2+, Cu2+ and the detergent SDS were strong inhibitors, while Mn2+ ions and dithiothreitol were stimulators of the enzyme activity. The enzyme was specific for xylans, showing higher specific activity on birchwood xylan. The Michaelis-Menten constant (K-m) for birchwood xylan was estimated to be 2.3 mg ml(-1) while maximal velocity (V-max) was 233.1 mumol mg(-1) min(-1) of protein. The hydrolysis of oat spell xylan released only xylooligosaccharides. Published by Elsevier Ltd. | en |
dc.format.extent | 359-364 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | Aspergillus versicolor | pt |
dc.subject | xylanase | pt |
dc.subject | endoxylanase | pt |
dc.subject | enzyme purification | pt |
dc.title | Production, purification and characterization of a minor form of xylanase from Aspergillus versicolor | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, Brazil | - |
dc.identifier.doi | 10.1016/j.procbio.2004.01.010 | - |
dc.identifier.wos | WOS:000224162000047 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Process Biochemistry | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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