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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/20172
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dc.contributor.authorGusmao, Desiely S.-
dc.contributor.authorSantos, Adao V.-
dc.contributor.authorMarini, Danyelle C.-
dc.contributor.authorBacci Junior, Mauricio-
dc.contributor.authorBerbert-Molina, Marilia A.-
dc.contributor.authorLemos, Francisco Jose A.-
dc.date.accessioned2013-09-30T18:47:32Z-
dc.date.accessioned2014-05-20T13:56:26Z-
dc.date.accessioned2016-10-25T17:05:46Z-
dc.date.available2013-09-30T18:47:32Z-
dc.date.available2014-05-20T13:56:26Z-
dc.date.available2016-10-25T17:05:46Z-
dc.date.issued2010-09-01-
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2010.04.011-
dc.identifier.citationActa Tropica. Amsterdam: Elsevier B.V., v. 115, n. 3, p. 275-281, 2010.-
dc.identifier.issn0001-706X-
dc.identifier.urihttp://hdl.handle.net/11449/20172-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/20172-
dc.description.abstractIn this work we show that the lumen of Aedes aegypti midgut is highly colonized by bacteria that were identified by culture-dependent and culture-independent methods. rDNA sequences obtained were compared with those from GenBank and the main bacterial genera identified were: Serratia, Klebsiella, Asaia, Bacillus, Enterococcus, Enterobacter, Kluyvera and Pantoea. All genera were identified in midgut except Enterobacter that was observed only in eggs. Asaia and Pantoea were also identified in eggs and ovary, respectively. In addition two yeast genera were observed in A. aegypti: Pichia isolated from midgut and Candida identified in midgut and ovary. The genus Serratia was dominant in all isolation assays representing 54.5% of the total of microorganisms. Thirty-nine and 24 bacterial clones were successfully obtained from midguts 24 and 48 h after blood feeding (ABF), respectively. The majority of clones obtained were from Serratia sp. (48.7% and 50% for 24 and 48 h ABF, respectively). Light microscopy showed that bacteria were located preferentially in the posterior midgut, around the blood meal and associated with peritrophic matrix. Scanning electron microscopy images showed a high number of bacteria in midgut during blood digestion and the peak of bacterial enumeration was reached 48 h ABF, stage in which lumen was almost totally occupied by bacteria that were also interacting with epithelial microvilli. Our results show the dynamics of microbial colonization and their distribution in midgut during blood digestion. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent275-281-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectAedes aegyptien
dc.subjectMidguten
dc.subjectBacterial colonizationen
dc.subjectSerratiaen
dc.titleCulture-dependent and culture-independent characterization of microorganisms associated with Aedes aegypti (Diptera: Culicidae) (L.) and dynamics of bacterial colonization in the midguten
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)-
dc.contributor.institutionInst Fed Educ Ciência & Tecnol Fluminense IFF-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUENF, Lab Biotecnol, BR-28013602 Campos Dos Goytacazes, RJ, Brazil-
dc.description.affiliationInst Fed Educ Ciência & Tecnol Fluminense IFF, BR-28030130 Campos Dos Goytacazes, RJ, Brazil-
dc.description.affiliationFac Integradas Maria Imaculada, BR-13840040 Mogi Guacu, SP, Brazil-
dc.description.affiliationUniv Estadual Paulista, UNESP, Lab Evolucao Mol, BR-13506900 Rio Claro, SP, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Lab Evolucao Mol, BR-13506900 Rio Claro, SP, Brazil-
dc.identifier.doi10.1016/j.actatropica.2010.04.011-
dc.identifier.wosWOS:000279509000016-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofActa Tropica-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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