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- Purification of Candida guilliermondii and Pichia ohmeri killer toxin as an active agent against Penicillium expansum
- Universidade Estadual de Londrina (UEL)
- Meijo Univ
- Universidade Estadual Paulista (UNESP)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- JICA (Japan International Cooperation Agency)
- An antifungal assay with cell-free culture supernatant of Pichia ohmeri 158 and Candida guilliermondii P3 was tested against Penicillium expansum strain #2 at 25C by measuring hyphal length and percentage conidia germination. C. guilliermondii was more effective against P. expansum conidia germination (58.15% inhibition), while P. ohmeri showed higher inhibition of mycelial growth (66.17%), indicating a probable mechanism associated with killer activity. This killer toxin (molecular mass 3 kDa) was partially purified by normal phase HPLC, using TSKgel Amide-80 analytical and preparative columns. Compared with crude extract, the killer toxin eluted from the post analytical column significantly inhibited P. expansum:% inhibition rose from 42.16 to 90.93% (C. guilliermondii) and 39.32 to 91.12% (P. ohmeri) (p 0.05). The one-step purification process was adequate in isolating killer toxin from culture supernatant and also increased anti-Penicillium activity.
- Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment. Abingdon: Taylor & Francis Ltd, v. 26, n. 1, p. 73-81, 2009.
- Taylor & Francis Ltd
- Penicillium expansum
- antagonist yeasts
- killer toxin
- Biological control
- Acesso restrito
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