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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/21402
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dc.contributor.authorArcuri, Helen A.-
dc.contributor.authorApponi, Luciano H.-
dc.contributor.authorValentini, Sandro R.-
dc.contributor.authorDurigon, Edison L.-
dc.contributor.authorAzevedo, Walter F. de-
dc.contributor.authorFossey, Marcelo Andrés-
dc.contributor.authorRahal, Paula-
dc.contributor.authorde Souza, Fatima P.-
dc.date.accessioned2014-05-20T14:00:30Z-
dc.date.accessioned2016-10-25T17:08:02Z-
dc.date.available2014-05-20T14:00:30Z-
dc.date.available2016-10-25T17:08:02Z-
dc.date.issued2008-12-01-
dc.identifierhttp://dx.doi.org/10.1016/j.pep.2008.08.005-
dc.identifier.citationProtein Expression and Purification. San Diego: Academic Press Inc. Elsevier B.V., v. 62, n. 2, p. 146-152, 2008.-
dc.identifier.issn1046-5928-
dc.identifier.urihttp://hdl.handle.net/11449/21402-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/21402-
dc.description.abstractThe Human Respiratory Syncytial Virus (HRSV) fusion protein (F) was expressed in Escherichia call BL21A using the pET28a vector at 37 degrees C. The protein was purified from the soluble fraction using affinity resin. The structural quality of the recombinant fusion protein and the estimation of its secondary structure were obtained by circular dichroism. Structural models of the fusion protein presented 46% of the helices in agreement with the spectra by circular dichroism analysis. There are only few studies that succeeded in expressing the HRSV fusion protein in bacteria. This is a report on human fusion protein expression in E. call and structure analysis, representing a step forward in the development of fusion protein F inhibitors and the production of antibodies. (c) 2008 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent146-152-
dc.language.isoeng-
dc.publisherAcademic Press Inc. Elsevier B.V.-
dc.sourceWeb of Science-
dc.subjectFusion proteinen
dc.subjectPurificationen
dc.subjectDichroism analysisen
dc.subjectMolecular modelingen
dc.subjectExpressionen
dc.titleExpression and purification of human respiratory syncytial virus recombinant fusion proteinen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionPontifícia Universidade Católica do Rio Grande do Sul (PUCRS)-
dc.description.affiliationUNESP, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUNESP, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil-
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, BR-05508900 São Paulo, Brazil-
dc.description.affiliationPUCRGS, Fac Ciencias Biol, BR-90619900 Porto Alegre, RS, Brazil-
dc.description.affiliationUnespUNESP, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose do Rio Preto, SP, Brazil-
dc.description.affiliationUnespUNESP, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 02/08461-2-
dc.description.sponsorshipIdCNPq: 150358/2003-2-
dc.identifier.doi10.1016/j.pep.2008.08.005-
dc.identifier.wosWOS:000260598900002-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofProtein Expression and Purification-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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