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http://acervodigital.unesp.br/handle/11449/21960
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DC Field | Value | Language |
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dc.contributor.author | Watanabe, L. | - |
dc.contributor.author | Nirthanan, S. | - |
dc.contributor.author | Rajaseger, G. | - |
dc.contributor.author | Polikarpov, I | - |
dc.contributor.author | Kini, R. M. | - |
dc.contributor.author | Arni, R. K. | - |
dc.date.accessioned | 2014-05-20T14:02:18Z | - |
dc.date.accessioned | 2016-10-25T17:09:01Z | - |
dc.date.available | 2014-05-20T14:02:18Z | - |
dc.date.available | 2016-10-25T17:09:01Z | - |
dc.date.issued | 2002-10-01 | - |
dc.identifier | http://dx.doi.org/10.1107/S0907444902011022 | - |
dc.identifier.citation | Acta Crystallographica Section D-biological Crystallography. Hoboken: Wiley-blackwell, v. 58, p. 1879-1881, 2002. | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | http://hdl.handle.net/11449/21960 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/21960 | - |
dc.description.abstract | Bucain is a three-finger toxin, structurally homologous to snake-venom muscarinic toxins, from the venom of the Malayan krait Bungarus candidus. These proteins have molecular masses of approximately 6000-8000 da and encompass the potent curaremimetic neurotoxins which confer lethality to Elapidae and Hydrophidae venoms. Bucain was crystallized in two crystal forms by the hanging-drop vapour-diffusion technique in 0.1 M sodium citrate pH 5.6, 15% PEG 4000 and 0.15 M ammonium acetate. Form I crystals belong to the monoclinic system space group C2, with unit-cell parameters a = 93.73, b = 49.02, c = 74.09 Angstrom, beta = 111.32degrees, and diffract to a nominal resolution of 1.61 Angstrom. Form II crystals also belong to the space group C2, with unit-cell parameters a = 165.04, b = 49.44, c = 127.60 Angstrom, beta = 125.55degrees, and diffract to a nominal resolution of 2.78 Angstrom. The self-rotation function indicates the presence of four and eight molecules in the crystallographic asymmetric unit of the form I and form II crystals, respectively. Attempts to solve these structures by molecular-replacement methods have not been successful and a heavy-atom derivative search has been initiated. | en |
dc.format.extent | 1879-1881 | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-Blackwell | - |
dc.source | Web of Science | - |
dc.title | Crystallization and preliminary X-ray analysis of bucain, a novel toxin from the Malayan krait Bungarus candidus | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.contributor.institution | Natl Univ Singapore | - |
dc.contributor.institution | Lab Nacl Luz Sincrotron | - |
dc.description.affiliation | UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | - |
dc.description.affiliation | Natl Univ Singapore, Fac Med, Singapore 117548, Singapore | - |
dc.description.affiliation | Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 119260, Singapore | - |
dc.description.affiliation | Lab Nacl Luz Sincrotron, Campinas, SP, Brazil | - |
dc.description.affiliationUnesp | UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | - |
dc.identifier.doi | 10.1107/S0907444902011022 | - |
dc.identifier.wos | WOS:000178248100027 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Acta Crystallographica Section D: Biological Crystallography | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
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