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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/22182
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dc.contributor.authorRibeiro Leite, Rodrigo Simoes-
dc.contributor.authorGomes, Eleni-
dc.contributor.authorda Silva, Roberto-
dc.date.accessioned2014-05-20T14:02:57Z-
dc.date.accessioned2016-10-25T17:09:26Z-
dc.date.available2014-05-20T14:02:57Z-
dc.date.available2016-10-25T17:09:26Z-
dc.date.issued2007-07-01-
dc.identifierhttp://dx.doi.org/10.1016/j.procbio.2007.05.003-
dc.identifier.citationProcess Biochemistry. Oxford: Elsevier B.V., v. 42, n. 7, p. 1101-1106, 2007.-
dc.identifier.issn1359-5113-
dc.identifier.urihttp://hdl.handle.net/11449/22182-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/22182-
dc.description.abstractThe thermophilic fungus Thermoascus aurantiacus 179-5 and the mesophilic Aureobasidium pullulans ER-16 were cultivated in corn-cob by solid state fermentation for P-glucosidase production. After fermentation both enzymes were purified. The beta-glucosidases produced by the strains A. pullulans and T aurantiacus were most active at pH 4.0-4.5 and 4.5, with apparent optimum temperatures at 80 and 75 degrees C, respectively. Surprisingly, the enzyme produced by the mesophilic A. pullulans was stable over a wider range of pH (4.5-9.5 against 4.5-6.5) and more thermostable (98% after 1 h at 75 degrees C against 98% after 1 h at 70 degrees C) than the enzyme from the thermophilic T. aurantiacus. The t((1/2)) at 80 degrees C were 90 and 30 min for A. pullulans and T. aurantiacus, respectively. beta-Glucosidase thermoinactivation followed first-order kinetics and the energies of denaturation were 414 and 537 kJ mol(-1) for T. aurantiacus and A. pullulans, respectively. The result showed that beta-glucosidase obtained from the mesophilic A. pullulans is more stable than that obtained from the thermophilic T. aurantiacus. (C) 2007 Elsevier Ltd. All rights reserved.en
dc.format.extent1101-1106-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectbeta-glucosidasept
dc.subjectAureobasidium pullulanspt
dc.subjectThermoascus aurantiacuspt
dc.subjectthermoinactivationpt
dc.subjectthermostabilitypt
dc.subjectthermophilicpt
dc.titleCharacterization and comparison of thermostability of purified beta-glucosidases from a mesophilic Aureobasidium pullulans and a thermophilic Thermoascus aurantiacusen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniv Estadual Paulista, Lab Bioquim & Microbiol Aplicada, IBILCE Inst Biociencias Letras & Ciências Exatas, BR-15054000 São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Lab Bioquim & Microbiol Aplicada, IBILCE Inst Biociencias Letras & Ciências Exatas, BR-15054000 São Paulo, Brazil-
dc.identifier.doi10.1016/j.procbio.2007.05.003-
dc.identifier.wosWOS:000248470800009-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofProcess Biochemistry-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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