Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/22182
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ribeiro Leite, Rodrigo Simoes | - |
dc.contributor.author | Gomes, Eleni | - |
dc.contributor.author | da Silva, Roberto | - |
dc.date.accessioned | 2014-05-20T14:02:57Z | - |
dc.date.accessioned | 2016-10-25T17:09:26Z | - |
dc.date.available | 2014-05-20T14:02:57Z | - |
dc.date.available | 2016-10-25T17:09:26Z | - |
dc.date.issued | 2007-07-01 | - |
dc.identifier | http://dx.doi.org/10.1016/j.procbio.2007.05.003 | - |
dc.identifier.citation | Process Biochemistry. Oxford: Elsevier B.V., v. 42, n. 7, p. 1101-1106, 2007. | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | http://hdl.handle.net/11449/22182 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/22182 | - |
dc.description.abstract | The thermophilic fungus Thermoascus aurantiacus 179-5 and the mesophilic Aureobasidium pullulans ER-16 were cultivated in corn-cob by solid state fermentation for P-glucosidase production. After fermentation both enzymes were purified. The beta-glucosidases produced by the strains A. pullulans and T aurantiacus were most active at pH 4.0-4.5 and 4.5, with apparent optimum temperatures at 80 and 75 degrees C, respectively. Surprisingly, the enzyme produced by the mesophilic A. pullulans was stable over a wider range of pH (4.5-9.5 against 4.5-6.5) and more thermostable (98% after 1 h at 75 degrees C against 98% after 1 h at 70 degrees C) than the enzyme from the thermophilic T. aurantiacus. The t((1/2)) at 80 degrees C were 90 and 30 min for A. pullulans and T. aurantiacus, respectively. beta-Glucosidase thermoinactivation followed first-order kinetics and the energies of denaturation were 414 and 537 kJ mol(-1) for T. aurantiacus and A. pullulans, respectively. The result showed that beta-glucosidase obtained from the mesophilic A. pullulans is more stable than that obtained from the thermophilic T. aurantiacus. (C) 2007 Elsevier Ltd. All rights reserved. | en |
dc.format.extent | 1101-1106 | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.source | Web of Science | - |
dc.subject | beta-glucosidase | pt |
dc.subject | Aureobasidium pullulans | pt |
dc.subject | Thermoascus aurantiacus | pt |
dc.subject | thermoinactivation | pt |
dc.subject | thermostability | pt |
dc.subject | thermophilic | pt |
dc.title | Characterization and comparison of thermostability of purified beta-glucosidases from a mesophilic Aureobasidium pullulans and a thermophilic Thermoascus aurantiacus | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Univ Estadual Paulista, Lab Bioquim & Microbiol Aplicada, IBILCE Inst Biociencias Letras & Ciências Exatas, BR-15054000 São Paulo, Brazil | - |
dc.description.affiliationUnesp | Univ Estadual Paulista, Lab Bioquim & Microbiol Aplicada, IBILCE Inst Biociencias Letras & Ciências Exatas, BR-15054000 São Paulo, Brazil | - |
dc.identifier.doi | 10.1016/j.procbio.2007.05.003 | - |
dc.identifier.wos | WOS:000248470800009 | - |
dc.rights.accessRights | Acesso restrito | - |
dc.relation.ispartof | Process Biochemistry | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.