You are in the accessibility menu

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPereira, Cristiane Aparecida-
dc.contributor.authorRomeiro, Rogerio Lima-
dc.contributor.authorBorges Pereira Costa, Anna Carolina-
dc.contributor.authorSilva Machado, Ana Karina-
dc.contributor.authorJunqueira, Juliana Campos-
dc.contributor.authorCardoso Jorge, Antonio Olavo-
dc.identifier.citationLasers In Medical Science. London: Springer London Ltd, v. 26, n. 3, p. 341-348, 2011.-
dc.description.abstractThe purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using methylene blue as photosensitizer and low-power laser irradiation on the viability of single-, dual-, and three-species biofilms formed by C. albicans, S. aureus, and S. mutans. Biofilms were grown in acrylic discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (10(6) cells/ml) and incubated for 5 days. on the fifth day, the effects of the methylene blue (MB) photosensitizer at a concentration of 0.1 mg/ml for 5 min and InGaAlP laser (660 nm) for 98 s, alone and conjugated were evaluated. Next, the discs were placed in tubes with sterile physiological solution [0.9% sodium chloride (NaCl)] and sonicated for to disperse the biofilms. Ten-fold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then the numbers CFU/ml (log(10)) were counted and analyzed statistically (ANOVA, Tukey test, p < 0.05). Scanning electron microscopy (SEM) on discs treated with PDI and control biofilms groups was performed. Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by MB dye. Reductions (log(10)) of single-species biofilms were greater (2.32-3.29) than the association of biofilms (1.00-2.44). Scanning electron microscopy micrographs suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. The results showed that PDI mediated by MB dye, might be a useful approach for the control of oral biofilms.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.publisherSpringer London Ltd-
dc.sourceWeb of Science-
dc.subjectCandida albicansen
dc.subjectStaphylococcus aureusen
dc.subjectStreptococcus mutansen
dc.subjectPhotodynamic inactivationen
dc.titleSusceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro studyen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent Sao Jose Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Dimas, SP, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent Sao Jose Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Dimas, SP, Brazil-
dc.description.sponsorshipIdFAPESP: 09/52048-1-
dc.description.sponsorshipIdFAPESP: 10/00879-4-
dc.description.sponsorshipIdFAPESP: 09/12005-1-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofLasers in Medical Science-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.