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dc.contributor.authorGodinho Vilela, Simone Furgeri-
dc.contributor.authorJunqueira, Juliana Campos-
dc.contributor.authorBarbosa, Junia Oliveira-
dc.contributor.authorMajewski, Marta-
dc.contributor.authorMunin, Egberto-
dc.contributor.authorCardoso Jorge, Antonio Olavo-
dc.identifier.citationArchives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 6, p. 704-710, 2012.-
dc.description.abstractObjectives: The organization of biofilms in the oral cavity gives them added resistance to antimicrobial agents. The action of phenothiazinic photosensitizers on oral biofilms has already been reported. However, the action of the malachite green photosensitizer upon biofilm-organized microorganisms has not been described. The objective of the present work was to compare the action of malachite green with the phenothiazinic photosensitizers (methylene blue and toluidine blue) on Staphylococcus aureus and Escherichia coli biofilms.Methods: The biofilms were grown on sample pieces of acrylic resin and subjected to photodynamic therapy using a 660-nm diode laser and photosensitizer concentrations ranging from 37.5 to 3000 mu M. After photodynamic therapy, cells from the biofilms were dispersed in a homogenizer and cultured in Brain Heart Infusion broth for quantification of colony-forming units per experimental protocol. For each tested microorganism, two control groups were maintained: one exposed to the laser radiation without the photosensitizer (L+PS-) and other treated with the photosensitizer without exposure to the red laser light (L-PS+). The results were subjected to descriptive statistical analysis.Results: The best results for S. aureus and E. coli biofilms were obtained with photosensitizer concentrations of approximately 300 mu M methylene blue, with microbial reductions of 0.8-1.0 log(10); 150 mu M toluidine blue, with microbial reductions of 0.9-1.0 log(10); and 3000 mu M malachite green, with microbial reductions of 1.6-4.0 log(10).Conclusion: Greater microbial reduction was achieved with the malachite green photosensitizer when used at higher concentrations than those employed for the phenothiazinic dyes. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.publisherPergamon-Elsevier B.V. Ltd-
dc.sourceWeb of Science-
dc.subjectPhotodynamic therapyen
dc.subjectMethylene blueen
dc.subjectToluidine blueen
dc.subjectMalachite greenen
dc.subjectOral microbiologyen
dc.titlePhotodynamic inactivation of Staphylococcus aureus and Escherichia coli biofilms by malachite green and phenothiazine dyes: An in vitro studyen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade Camilo Castelo Branco (UNICASTELO)-
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent, Microbiol Lab, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP, Brazil-
dc.description.affiliationCamilo Castelo Branco Univ UNICASTELO, BR-12247004 Sao Jose Dos Campos, SP, Brazil-
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent, Microbiol Lab, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP, Brazil-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofArchives of Oral Biology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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