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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/2415
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dc.contributor.authordos Santos, CBD-
dc.contributor.authorde Carvalho, ACFB-
dc.contributor.authorRagozo, AMA-
dc.contributor.authorSoares, R. M.-
dc.contributor.authorAmaku, M.-
dc.contributor.authorYai, LEO-
dc.contributor.authorDubey, J. P.-
dc.contributor.authorGennari, S. M.-
dc.date.accessioned2014-05-20T13:15:12Z-
dc.date.accessioned2016-10-25T16:36:29Z-
dc.date.available2014-05-20T13:15:12Z-
dc.date.available2016-10-25T16:36:29Z-
dc.date.issued2005-08-10-
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2005.04.039-
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 131, n. 3-4, p. 207-211, 2005.-
dc.identifier.issn0304-4017-
dc.identifier.urihttp://hdl.handle.net/11449/2415-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/2415-
dc.description.abstractToxoplasma gondii infection is widely prevalent in humans in Brazil. Among the food animals, pigs are considered the most important meat source of T. gondii for infection in humans. In the present study, we report the first isolation of viable T. gondii from finishing pigs in Brazil. Antibodies to T. gondii were found in 49 (17%) of 286 pigs prior slaughter using the modified agglutination test (MAT) at a serum dilution of 1:25. Attempts were made to isolate T. gondii from 28 seropositive pigs. Samples of heart, brain, and tongue from each pig were pooled, digested in acid pepsin, and bioassayed in five mice per pig. Viable T. gondii was isolated from seven pigs; all isolates were lethal for mice. Restriction fragment length polymorphism on products of SAG2 locus amplified by PCR revealed that two isolates were Type I and five were Type III. The results indicate that phenotypically and genetically T. gondii isolates from pigs from Brazil are distinct from isolates of T gondii from pigs in the USA. (c) 2005 Elsevier B.V. All rights reserved.en
dc.format.extent207-211-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.sourceWeb of Science-
dc.subjectToxoplasma gondiipt
dc.subjectpigspt
dc.subjectgenotypingpt
dc.subjectBrazilpt
dc.subjectbioassaypt
dc.titleFirst isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazilen
dc.typeoutro-
dc.contributor.institutionUSDA ARS-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.contributor.institutionCtr Controle Zoonoses Prefeitura Municipio Sao Pa-
dc.description.affiliationUSDA ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, BARC E, Beltsville, MD 20705 USA-
dc.description.affiliationUniv Estadual Paulista, Dept Med Vet Prevent, Fac Ciências Agrarias & Vet, Jaboticabal, SP, Brazil-
dc.description.affiliationUniv São Paulo, Dept Med Vet Prevent & Saúde Anim, Fac Med Vet & Zootecn, BR-05508000 São Paulo, Brazil-
dc.description.affiliationCtr Controle Zoonoses Prefeitura Municipio Sao Pa, São Paulo, Brazil-
dc.description.affiliationUnespUniv Estadual Paulista, Dept Med Vet Prevent, Fac Ciências Agrarias & Vet, Jaboticabal, SP, Brazil-
dc.identifier.doi10.1016/j.vetpar.2005.04.039-
dc.identifier.wosWOS:000230774300004-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofVeterinary Parasitology-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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