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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/25349
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dc.contributor.authorTrevisan, Henrique Celso-
dc.contributor.authorMedeiros, João Batista de-
dc.contributor.authorLisboa, Helen Cristina Fávero-
dc.date.accessioned2014-05-20T14:17:51Z-
dc.date.accessioned2016-10-25T17:40:11Z-
dc.date.available2014-05-20T14:17:51Z-
dc.date.available2016-10-25T17:40:11Z-
dc.date.issued2006-07-01-
dc.identifierhttp://dx.doi.org/10.1590/S0100-40422006000400040-
dc.identifier.citationQuímica Nova. Sociedade Brasileira de Química, v. 29, n. 4, p. 865-867, 2006.-
dc.identifier.issn0100-4042-
dc.identifier.urihttp://hdl.handle.net/11449/25349-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/25349-
dc.description.abstractA simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
dc.format.extent865-867-
dc.language.isopor-
dc.publisherSociedade Brasileira de Química-
dc.sourceSciELO-
dc.subjectporcine liver esteraseen
dc.subjectPLEen
dc.subjectbiocatalysisen
dc.titleExtração de esterase de fígado suíno (PLE)pt
dc.title.alternativePig liver esterase (PLE) extractionen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho Instituto de Química Departamento de Bioquímica e Tecnologia Química-
dc.description.affiliationUnespUniversidade Estadual Paulista Julio de Mesquita Filho Instituto de Química Departamento de Bioquímica e Tecnologia Química-
dc.identifier.doi10.1590/S0100-40422006000400040-
dc.identifier.scieloS0100-40422006000400040-
dc.rights.accessRightsAcesso aberto-
dc.identifier.fileS0100-40422006000400040.pdf-
dc.relation.ispartofQuímica Nova-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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