Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/25349
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Trevisan, Henrique Celso | - |
dc.contributor.author | Medeiros, João Batista de | - |
dc.contributor.author | Lisboa, Helen Cristina Fávero | - |
dc.date.accessioned | 2014-05-20T14:17:51Z | - |
dc.date.accessioned | 2016-10-25T17:40:11Z | - |
dc.date.available | 2014-05-20T14:17:51Z | - |
dc.date.available | 2016-10-25T17:40:11Z | - |
dc.date.issued | 2006-07-01 | - |
dc.identifier | http://dx.doi.org/10.1590/S0100-40422006000400040 | - |
dc.identifier.citation | Química Nova. Sociedade Brasileira de Química, v. 29, n. 4, p. 865-867, 2006. | - |
dc.identifier.issn | 0100-4042 | - |
dc.identifier.uri | http://hdl.handle.net/11449/25349 | - |
dc.identifier.uri | http://acervodigital.unesp.br/handle/11449/25349 | - |
dc.description.abstract | A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C. | en |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
dc.format.extent | 865-867 | - |
dc.language.iso | por | - |
dc.publisher | Sociedade Brasileira de Química | - |
dc.source | SciELO | - |
dc.subject | porcine liver esterase | en |
dc.subject | PLE | en |
dc.subject | biocatalysis | en |
dc.title | Extração de esterase de fígado suíno (PLE) | pt |
dc.title.alternative | Pig liver esterase (PLE) extraction | en |
dc.type | outro | - |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | - |
dc.description.affiliation | Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Química Departamento de Bioquímica e Tecnologia Química | - |
dc.description.affiliationUnesp | Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Química Departamento de Bioquímica e Tecnologia Química | - |
dc.identifier.doi | 10.1590/S0100-40422006000400040 | - |
dc.identifier.scielo | S0100-40422006000400040 | - |
dc.rights.accessRights | Acesso aberto | - |
dc.identifier.file | S0100-40422006000400040.pdf | - |
dc.relation.ispartof | Química Nova | - |
Appears in Collections: | Artigos, TCCs, Teses e Dissertações da Unesp |
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.