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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/2535
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dc.contributor.authorOliveira, L. Z.-
dc.contributor.authorArruda, R. P.-
dc.contributor.authorCeleghini, E. C. C.-
dc.contributor.authorde Andrade, A. F. C.-
dc.contributor.authorPerini, A. P.-
dc.contributor.authorResende, M. V.-
dc.contributor.authorMiguel, M. C. V.-
dc.contributor.authorLucio, A. C.-
dc.contributor.authorHossepian de Lima, V. F. M.-
dc.date.accessioned2014-05-20T13:15:22Z-
dc.date.accessioned2016-10-25T16:36:42Z-
dc.date.available2014-05-20T13:15:22Z-
dc.date.available2016-10-25T16:36:42Z-
dc.date.issued2012-02-01-
dc.identifierhttp://dx.doi.org/10.1111/j.1439-0272.2010.01096.x-
dc.identifier.citationAndrologia. Malden: Wiley-blackwell, v. 44, n. 1, p. 9-15, 2012.-
dc.identifier.issn0303-4569-
dc.identifier.urihttp://hdl.handle.net/11449/2535-
dc.identifier.urihttp://acervodigital.unesp.br/handle/11449/2535-
dc.description.abstractThe objective of this study was to evaluate the quality of bovine frozen-thawed sperm cells after Percoll gradient centrifugation. Frozen semen doses were obtained from six bulls of different breeds, including three taurine and three Zebu animals. Four ejaculates per bull were evaluated before and after discontinuous Percoll gradient centrifugation. Sperm motility was assessed by computer-assisted semen analysis and the integrity of the plasma and acrosomal membranes, as well as mitochondrial function, were evaluated using a combination of fluorescent probes propidium iodide, fluorescein isothiocyanate-conjugated Pisum sativum agglutinin and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide. The procedure of Percoll gradient centrifugation increased the percentage of total and progressive sperm motility, beat frequency, rectilinear motility, linearity and rapidly moving cells. In addition, the percentage of cells with intact plasma membrane and mitochondrial membrane potential was increased in post-centrifugation samples. However, the percentage of sperm cells with intact acrosomal membrane was markedly reduced. The method used selected the motile cells with intact plasma membrane and higher mitochondrial functionality in frozen-thawed bull semen, but processing, centrifugation and/or the Percoll medium caused damage to the acrosomal membrane.en
dc.language.isoeng-
dc.publisherWiley-Blackwell-
dc.sourceWeb of Science-
dc.subjectAcrosome integrityen
dc.subjectCryopreserved semenen
dc.subjectsperm selectionen
dc.titleEffects of discontinuous Percoll gradient centrifugation on the quality of bovine spermatozoa evaluated with computer-assisted semen analysis and fluorescent probes associationen
dc.typeoutro-
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)-
dc.contributor.institutionUniversidade de São Paulo (USP)-
dc.description.affiliationSão Paulo State Univ UNESP, Sch Agr Sci & Vet Med, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, Brazil-
dc.description.affiliationUniv São Paulo, Vet Med & Anim Sci Sch, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Pirassununga, SP, Brazil-
dc.description.affiliationUnespSão Paulo State Univ UNESP, Sch Agr Sci & Vet Med, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP, Brazil-
dc.identifier.doi10.1111/j.1439-0272.2010.01096.x-
dc.identifier.wosWOS:000298788600002-
dc.rights.accessRightsAcesso restrito-
dc.relation.ispartofAndrologia-
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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